中国临床药学杂志2024，Vol.33Issue(5)：370-375,6.DOI:10.19577/j.1007-4406.2024.05.008
基于TGF-β1/Smads信号通路探讨罗汉果甜苷抗肝纤维化的机制
Study of the regulatory mechanism of hepatic stellate cells by Mogroside based on TGF-β1/Smads signaling pathway
摘要
Abstract
AIM To investigate the mechanism of anti-liver fibrosis of mogroside(Mog)via TGF-β1/Smads signaling pathway.METHODS Hepatic stellate cell line HSC-T6 was cultured in vitro.HSC-T6 cells were treated with serum containing various volume concentrations of Mog(5％,10％and 20％).The proliferation ability,toxicity release and migration ability of HSC-T6 were detected.The protein and mRNA expression levels of Smad2,3,4,7 and TGF-β1 in HSC-T6 were detected.The expression level of α-SMA in cells was detected.RESULTS Compared with the model group,Mog-containing serum at concentrations of 5％,10％and 20％significantly reduced the number of HSC-T6 proliferation within 24 h(P＜0.05),without any significant toxicity(P＞0.05).The cell migration rates in the 5％,10％,and 20％Mog groups were significantly lower than those in the model group(P＜0.05).All concentrations of Mog-containing serum were able to inhibit the expression of TGF-β1,Smad2,3,7 at both mRNA and protein levels in HSC-T6(P＜0.05).The protein and mRNA expression levels of Smad4 were higher than those of the model group(P＜0.05).The expression of α-SMA,a key marker of liver fibrosis,was also significantly down-regulated(P＜0.05).CONCLUSION Mog can inhibit the activation of HSC-T6 by regulating the expression of TGF-β1/Smads signaling pathway-related proteins and mRNA,thus playing an anti-hepatic fibrosis role.关键词
罗汉果甜苷/肝纤维化/肝星状细胞/TGF-β1/Smads信号通路/含药血清Key words
mogroside/liver fibrosis/hepatic stellate cell/TGF-β1/Smads signaling pathway/drug-containing serum分类
社会科学引用本文复制引用
何晓璇,肖刚,罗陈亮,吴桂有,李悦,康碧倩,黄忠仕..基于TGF-β1/Smads信号通路探讨罗汉果甜苷抗肝纤维化的机制[J].中国临床药学杂志,2024,33(5):370-375,6.基金项目
广西自然科学基金青年基金项目(编号2013GXNSFBA019197) （编号2013GXNSFBA019197）
