中国免疫学杂志2024，Vol.40Issue(7)：1495-1500,1506,7.DOI:10.3969/j.issn.1000-484X.2024.07.025
聚乙二醇衍生物修饰的IL-12制备及体外评价
Preparation and in vitro evaluation of polyethylene glycol derivatives modified IL-12
摘要
Abstract
Objective:To prepare IL-12 modified with polyethylene glycol derivatives and determine its modification sites,and to evaluate it in vitro in terms of stability,hemogram recovery,anti-tumor effects and other aspects.Methods:IL-12 was modified with polyethylene glycol propionaldehyde and maleimide imide derivatives,and modified sites of two different derivatives were deter-mined by ultra performance liquid chromatography(UPLC).Recombinant human IL-12 and PEG-IL-12 were used to stimulate NK92 cells,activity and cytotoxicity of IFN-γ were evaluated by kits.CD34+was stimulated to evaluate its blood picture recovery potential.NK cell killing was stimulated to evaluate its effectiveness in improving immunity and anti-tumor effects.Results:Compared with recombinant human IL-12,PEG-IL-12 had higher stability,blood picture recovery potential and anti-tumor effects.Conclusion:PEG-IL-12 can effectively overcome many drawbacks of recombinant human IL-12,greatly improving possibility of its widespread applica-tion in clinical trials.关键词
聚乙二醇/IL-12/血象恢复/抗肿瘤Key words
PEG/IL-12/Hemogram recovery/Anti-tumor分类
医药卫生引用本文复制引用
刘艺,邹镇阳,李宗艳,韩峰,乔天奎,王建刚..聚乙二醇衍生物修饰的IL-12制备及体外评价[J].中国免疫学杂志,2024,40(7):1495-1500,1506,7.基金项目
国家科技重大专项"重大新药创制"(2017ZX09303017) （2017ZX09303017）
山东省重大科技创新工程项目(NO2018CXGC1404). （NO2018CXGC1404）
