中国人兽共患病学报2024，Vol.40Issue(6)：498-503,6.DOI:10.3969/j.issn.1002-2694.2024.00.076
艰难梭菌毒素B2型蛋白重组表达及活性分析
Expression and activity analysis of Clostridium difficile toxin B type 2
摘要
Abstract
This study was aimed at creating an engineered strain of Bacillus subtilis for efficient expression of biologically active type 2 toxin B(TcdB2)derived from a highly virulent strain of Clostridium difficile.The TcdB2 gene was cloned from ST1/RT027 strain genome DNA,incorporated into the PHT01 vector,and then transformed into B.subtilis strain WB800N for prokaryotic expression.Cell toxicity assays revealed that the recombinant TcdB2 exhibited cytotoxic effects in various cells.The engineered B.subtilis strain effectively expressed biologically active TcdB2,thus providing a basis for further exploration of the pathogenic mechanisms of highly virulent strains of C.difficile and establishing a foundation for potential vaccine can-didate targets.关键词
艰难梭菌/毒素B/枯草芽胞杆菌/表达/细胞毒性Key words
Clostridium difficile/toxin B/Bacillus subtilis/expression/cytotoxicity分类
医药卫生引用本文复制引用
林兴豪,张凯,王梦婕,杨鸣,顾汉阳,薛笑兰,罗永能,金大智,胡珲..艰难梭菌毒素B2型蛋白重组表达及活性分析[J].中国人兽共患病学报,2024,40(6):498-503,6.基金项目
Supported by the National Natural Science Foundation of China(No.8210030375)and the Foundation from Zhejiang Medical and Health Science and Technology(No.2021RC050). 国家自然科学基金(No.8210030375)与浙江省医药卫生科技项目(No.2021RC050)联合资助 （No.8210030375）
