中国人兽共患病学报2024，Vol.40Issue(6)：504-511,528,9.DOI:10.3969/j.issn.1002-2694.2024.00.087
我国新分离芒市病毒基因组特征与细胞感染特性研究
Genomic and cellular infection characteristics of a newly isolated Mangshi virus in China
摘要
Abstract
The genomic characteristics and cellular tropism of a Mangshi virus(MSV)isolated in China were investigated,thereby establishing a robust foundation for further research on the evolution and pathogenicity of MSV.The genome sequence of MSV strain V301/YNJH/2019 was obtained by next-generation sequencing,followed by phylogenetic tree construction and rearrangement assessment using software IQtree,RPD4,and Simplot.Viral proliferation was assessed in C6/36(Aedes albop-ictus),Vero(African green monkey kidney),and BHK(baby hamster kidney)cells.An initial epidemiological investigation of MSV in local cattle and goats was conducted using the serum neutralization test.The genome of MSV strain V301/YNJ H/2019 was 20623 bp in length,encompassing 12 segments of double-stranded RNA(Seg-1 to Seg-12).Sequence analysis confirmed genomic rearrangement of the Seg-1 and Seg-11 sequences,ex-hibiting high similarity to MSV isolated from lake sediment in China in 2022,while Seg-2 to Seg-10 and Seg-12 were most closely related to a MSV strain isolated from mosquitoes in China in 2013.The virus efficiently proliferated and induced sig-nificant cytopathic effects(CPE)in both C6/36 and BHK cells,but limited replication and no observable CPE in Vero cells.No detectable neutralizing antibodies against MSV were detected in 20 goat serum samples collected in Mangshi,while 2 of 20 bo-vine serum samples were positive with neutralizing antibody titers of 1:128 and 1:54.Whole genome sequencing revealed re-assortment events of the V301/YNJH/2019 strain,which is capable of infecting C6/36,BHK,and Vero cells.MSV infection was confirmed in cattle in Mangshi.关键词
芒市病毒/全基因组测序/基因重配/细胞感染特性/流行病学调查Key words
Mangshi virus/complete genome sequencing/reassortment/celltropism/epidemiological survey分类
农业科技引用本文复制引用
杨恒,李占鸿,肖雷,李卓然,谢佳蕊,廖德芳,高林,李华春..我国新分离芒市病毒基因组特征与细胞感染特性研究[J].中国人兽共患病学报,2024,40(6):504-511,528,9.基金项目
国家自然科学基金(No.31760744) （No.31760744）
国家公益性行业(农业)专项(No.201303035) （农业）
昆明学院引进人才科研项目(No.YJL2210) Supported by National Natural Science Foundation of China(No.31760744) （No.YJL2210）
Special Fund for Agro-scientific Research in the Public Interest(No.201303035) （No.201303035）
Scientific research project for talents introduced by Kunming University(No.YJL2210) （No.YJL2210）
