中国人兽共患病学报2024，Vol.40Issue(6)：512-519,8.DOI:10.3969/j.issn.1002-2694.2024.00.088
信鸽产酸克雷伯菌GS-BY-GG的分离鉴定
Isolation and identification of Klebsiella oxytoca GS-BY-GG from racing pigeons
摘要
Abstract
The biological characteristics and pathogenicity of Klebsiella oxytoca isolated from sick carrier pigeons in Gansu province were explored by morphological observations,biochemical testing,16S rRNA PCR analysis,and RNA sequencing.The drug resistance and pathogenicity of the isolated strains were studied by histopathological observation,drug susceptibility testing,and pathogenicity analysis.The livers,lungs,hearts,and other organs of the sick pigeons were bleeding.In addition,the livers were yellow and brittle,and the lungs were purulent.A Gram-negative,short,rod-shaped bacterium was successfully isolated from the sick pigeon.Pink,smooth,moist,and round colonies grew on MacConkey's agar.The result of the indigo matrix test was positive.The homology between the amplified 16S rRNA sequence and MN330093.1 was 100.00％,indicating that the sick pigeon was infected with K.oxytoca.The strain was named GS-BY-GG.K.Oxytoca GS-BY-GG was resistant to 10 drugs,including penicillin,ampicillin,and furazolidone,and sensitive to 5 others,which included florfenicol,meropenem,and gentamicin.Histopathological observation showed bleeding in multiple organs.The liver cells were irregu-larly arranged with brown-yellow pigmentation.Extensive cell necrosis and exfoliation were observed in the trachea and mucosal epithelium,with inflammatory cell infiltration in the mucosal layer.The isolates were highly pathogenic in specific pathogen-free chickens.These findings provide support for the clinical diagnosis and control of K.oxytoca GS-BY-GG.关键词
产酸克雷伯菌/信鸽/分离鉴定/耐药情况/组织病理学Key words
Klebsiella oxytoca/racing pigeon/isolation and identification/drug resistance/histopathology分类
医药卫生引用本文复制引用
李韦,李赟辉,金有顺,巴旭丽,张淮瑜,韩涛,李兆才,周继章..信鸽产酸克雷伯菌GS-BY-GG的分离鉴定[J].中国人兽共患病学报,2024,40(6):512-519,8.基金项目
国家重点研发计划专项(No.2022YFC2304001) Supported by the National Key Research and Development Program of China(No.2022YFC2304001) （No.2022YFC2304001）
