中国人兽共患病学报2024，Vol.40Issue(6)：529-536,8.DOI:10.3969/j.issn.1002-2694.2024.00.091
LAG3分子对泡球蚴感染小鼠肝脏B淋巴细胞亚群及其功能的影响
Effect of LAG3 molecule on B lymphocyte subsets and its function in the liver of mice infected with Echinococcus multilocularis
摘要
Abstract
This study was aimed at investigating the effect of lymphocyte activation gene-3(LAG3)on liver B lymphocyte subsets and their functions in WT and LAG3-KO mice infected with Echinococcus multilocularis(E.multilocularis).In a mouse model of E.multilocularis infection,the expression and localization of CD19 and α-SMA in liver were detected by immu nohistochemistry.CD80,CD86 and MHC-Ⅱ molecules expressed on B cells and their subsets in mice liver were detected by flow cytometry.After 12 weeks of infection,the area and percentage of CD19 in LAG3-KO group was slightly higher than that in WT group,but the difference was not statistically(t=-1.241、-1.237,P＞0.05).The area and percentage of a-SMA in LAG3-KO group was higher than that in WT group(t=-3.224、-3.227,P＜0.05).The proportion of CD80 and MHC-Ⅱ molecules expressed on liver B cells in LAG3-KO group was up-regulated(t=-2.379,-3.321,P＜0.05).The percentage of liver B2 cells in LAG3-KO group was higher than that in WT group(t=-2.695,P＜0.05).The expression of CD80 on Blb cells in LAG3-KO group was significantly up-regulated(t=-5.315,P＜0.001).The proportion of CD80 of B2 cells in LAG3-KO group was lower than that in WT group(t=2.806,P＜0.05).The expression of MHC-Ⅱ molecule in B2 cells in LAG3-KO group was up-regulated(t=-4.227,P＜0.01).It is suggested that LAG3 molecules affected the B cell subsets and func-tion of mouse liver in the middle stage of E.multilocularis infection,especially B2 lymphocytes.LAG3 molecule exerted an in-hibitory effect on the activation of B cells and the expression of MHC-class Ⅱ molecules,suggesting that it may be involved in B cell exhaustion caused by E.multilocularis.关键词
泡球蚴/淋巴细胞活化基因-3/B细胞/B细胞亚群Key words
Echinococcus multilocularis/LAG3/B cells/B cell subsets分类
医药卫生引用本文复制引用
郑旭然,王慧,张传山,李静,邓冰清,康雪娇,李寅时,阿比旦·艾尼瓦尔,余倩,孜比姑·肉素,阿迪莱·多力坤,王茂林..LAG3分子对泡球蚴感染小鼠肝脏B淋巴细胞亚群及其功能的影响[J].中国人兽共患病学报,2024,40(6):529-536,8.基金项目
国家自然科学基金(No.82060370,No.82302561,No.82372279)、新疆维吾尔自治区自然科学基金(No.2022D01E51,No.2022D01D60)、新疆地区高发疾病研究教育部重点实验室开放课题资助项目(No.2023B03) Supported by the National Natural Science Foundation of China(No.82060370,No.82302561,No.82372279),the Natural Sci-ence Foundation of Xinjiang Uygur Autonomous Region(No.2022D01E51,No.2022D01D60),and the Laboratory of High Inci-dence Disease Research in Xinjiang Ministry of Education(No.2023B03) （No.82060370,No.82302561,No.82372279）
