中国乳业Issue(7)：49-53,5.DOI:10.12377/1671-4393.24.07.09
牛病毒性腹泻病毒与冠状病毒混合感染的PCR诊断
PCR Diagnosis of Mixed Infection of Bovine Viral Diarrhea Virus and Coronavirus
李云龙 1李召英2
作者信息
- 1. 山东省临沂市沂水县农业综合执法大队,山东临沂 276400
- 2. 沂水县动物疫病预防控制中心,山东临沂 276400
- 折叠
摘要
Abstract
[Objective]To establish a PCR diagnostic method for mixed infection of Bovine Viral Diarrhea Virus(BVDV)and Bovine Coronavirus(BCoV).[Method]98 cows infected with BVDV and BCoV in a dairy farm in Shandong were selected as the research subjects.Their feces and tissues were collected and virus genomic RNA was extracted.After reverse transcription,primers were designed for PCR detection,and the detection status,specificity and repeatability were analyzed.[Result]40 cases were infected with BVDV and BCoV(40.82%),including 33 fecal samples(40.27%)and 7 tissue samples accounted for 7 cases(43.75%).There were 27 cases(27.55%)of BVDV single infection,including 21 fecal samples(25.61%)and 6 tissue samples(37.50%).There were 32 cases(32.65%)of BCoV single infection,including 28 fecal samples(34.15%)and 4 tissue samples(25.00%).[Conclusion]In this study,the PCR diagnostic method for the mixed infection of BVDV and BCoV was accurate,with good specificity and repeatability,which provided a reference for the correct diagnosis of these two viruses in clinical practice and laid a foundation for precise and scientific prevention and control in the future.关键词
牛病毒性腹泻病毒(BVDV)/冠状病毒(BCoV)/PCR/诊断Key words
Bovine Viral Diarrhea Virus(BVDV)/Bovine Coronavirus(BCoV)/PCR/diagnosis引用本文复制引用
李云龙,李召英..牛病毒性腹泻病毒与冠状病毒混合感染的PCR诊断[J].中国乳业,2024,(7):49-53,5.
