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不同浓度EMS诱变不结球白菜的效果分析

周玉彩 肖栋 李英 何盈 侯喜林

中国蔬菜Issue(7):42-49,8.
中国蔬菜Issue(7):42-49,8.DOI:10.19928/j.cnki.1000-6346.2024.0033

不同浓度EMS诱变不结球白菜的效果分析

Effects of Different Concentrations of EMS on Mutagenic Non-heading Chinese Cabbage

周玉彩 1肖栋 2李英 1何盈 1侯喜林1

作者信息

  • 1. 南京农业大学作物遗传与种质创新利用全国重点实验室,农业农村部华东地区园艺作物生物学与种质创制重点实验室,园艺作物种质创新与利用教育部工程研究中心,江苏南京 210095||南京苏曼等离子工程研究院有限公司,江苏南京 211162
  • 2. 南京农业大学作物遗传与种质创新利用全国重点实验室,农业农村部华东地区园艺作物生物学与种质创制重点实验室,园艺作物种质创新与利用教育部工程研究中心,江苏南京 210095
  • 折叠

摘要

Abstract

In order to create new germplasm and discover new genes,we explored the mutagenic effect of different concentrations of ethyl methane sulfonate(EMS)on non-heading Chinese cabbage.Four varieties of non-heading Chinese cabbage(HTC,XQC,JP241 and XWZ)were subjected to seed mutation treatment with EMS at concentrations of 0.4%,0.6%,0.8%,and seed germination and the variation of agronomic traits in the field were investigated.The results showed that the seed germination potential,germination rate and relative seedling establishment rate of the four varieties of non-heading Chinese cabbage gradually decreased with the increase of EMS concentration,and the differences between treatments were significant.EMS mutagenesis cause variations in the traits of leaf color,leaf shape,plant type and flowering organs of non-heading Chinese cabbage.The EMS-induced LD50 of different varieties of non-heading Chinese cabbage were different,and the types and frequencies of variation were also different.The LD50 of HTC was 0.8%,and the relative frequency of variation was 23.83%;the LD50 of XQC was 0.8%,and the relative frequency of variation was 17.61%;the LD50 of JP241 was 0.6%,and the relative frequency of variation was 38.45%;the LD50 of XWZ was 0.6%,and the relative variation frequency of 16.69%.As the concentration of EMS increased,the later the flowering time of the first flower of mutant non-heading Chinese cabbage,the fewer the number of rosette leaves,and the higher the male sterility rate.

关键词

不结球白菜/EMS/变异频率/变异类型

Key words

non-heading Chinese cabbage/ethyl methane sulfonate/variation frequency/variation type

引用本文复制引用

周玉彩,肖栋,李英,何盈,侯喜林..不同浓度EMS诱变不结球白菜的效果分析[J].中国蔬菜,2024,(7):42-49,8.

基金项目

中央高校基本科研业务费专项资金资助项目(CGPY2023009) (CGPY2023009)

中国蔬菜

OA北大核心CSTPCD

1000-6346

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