中国水产科学2024，Vol.31Issue(5)：537-545,9.DOI:10.12264/JFSC2024-0019
膨腹海马养殖群体遗传多样性
Genetic diversity analysis of cultured Hippocampus abdominalis population
摘要
Abstract
Hippocampus abdominalis are an economically important aquatic species in China.To understand the genetic structure of five populations of the main farmed distended seahorses in China[the third cultured stock in Shangdong province(SD),the first cultured stock in Fujian province(FJ1),the sixth cultured stock in Fujian province(FJ6),the 12th cultured stock in Tai Wan(TW),and breeding offspring of one Australian wild species(AS)],We developed six polymorphic microsatellite markers of the H.abdominalis,and used these 6 microsatellite markers for genetic analysis of five populations of H.abdominalis.The results showed that 63 alleles were detected by 6 pairs of primers in 144 samples.The number of alleles ranged from 8 to 14,with an average of 10.5 and PIC>0.25,indicating that all 6 microsatellite loci had high polymorphism.In addition,the observed heterozygosity and expected heterozygosity of the five populations were 0.6603 and 0.8104,respectively,indicating that the genetic diversity of the five populations was relatively rich.Further,the molecular variance analysis and genetic analysis was found that the genetic variation of the five populations mainly came from within the population,and a relatively high level of genetic distance was maintained among the 5 populations,among which the genetic distance between Taiwan(TW)and Fujian(FJ1)populations was the largest at 0.4529.In summary,there were genetic differences among the five H.abdominalis populations,and the genetic diversity of the populations was relatively rich and there was no decline in genetic diversity,indicating the 5 H.abdominalis populations could continue to be used for subsequent breeding.关键词
膨腹海马/微卫星标记/遗传分析/期望杂合度/遗传结构Key words
Hippocampus abdominalis/microsatellite loci/genetic analysis/expected heterozygosity/genetic structure分类
农业科技引用本文复制引用
郑乐云,吴锦辉,王庆,韦丽云,闫旭,卢柯宇,张敏琳,梁建韬,梁凯珊,何丽斌,甘松永..膨腹海马养殖群体遗传多样性[J].中国水产科学,2024,31(5):537-545,9.基金项目
国家重点研发计划项目(2022YFD2400502) （2022YFD2400502）
厦门市科技计划项目(2021FCX012501180007) （2021FCX012501180007）
广东省自然科学基金面上项目(2022A1515012505). （2022A1515012505）
