长江鲟早期发育阶段肠道菌群塑造机制OA北大核心CSTPCD
Mechanism of intestinal remodeling during the early development stage of Yangtze sturgeon,Acipenser dabryanus
为探究人工养殖条件下长江鲟(Acipenser dabryanus)早期发育过程中的肠道菌群塑造机制,通过高通量测序技术,开展长江鲟早期发育阶段肠道菌群与生物饵料水蚯蚓的微生物群落结构特征及相关性分析.结果显示,在门分类水平,长江鲟未开口摄食阶段肠道优势菌门以变形菌门(Proteobacteria)为主,开口摄食第 10～40 天期间以变形菌门和厚壁菌门(Firmicutes)为主.在属分类水平,未开口摄食至开口摄食第 20 天期间肠道菌群较为单一,潘多拉菌属(Pandoraea)占比绝对优势地位,占比超过 88%;开口摄食第 30 天,长江鲟肠道菌群发生重组,物种丰富度和多样性达到峰值,主要优势菌属变为柠檬酸杆菌属(Citrobacter)、乳球菌属(Lactococcus)、邻单胞菌属(Plesiomonas)、葡萄球菌属(Staphylococcus)、气单胞菌属(Aeromonas)和克雷伯氏菌属(Klebsiella).Procrustes分析显示,开口摄食第 40 天长江鲟肠道菌群与生物饵料水蚯蚓微生物群落组成趋于一致性,呈现极显著相关(P<0.01).进一步通过SourceTracker物种来源分析发现,长江鲟肠道微生物群落中开口摄食第10天有18.97%来源于生物饵料水蚯蚓,开口摄食第20天下降至0.15%,开口摄食第30天上升至51.22%,而在开口摄食第40天高达97.55%.本研究表明,采用生物饵料水蚯蚓投喂长江鲟,开口摄食第 30 天是其早期发育阶段肠道菌群的转折点,肠道菌群特征发生改变,由高度的保守性转变为良好的可塑性.继续投喂至第 40 天,其肠道菌群与生物饵料微生物群落表现出高度的一致性.研究结果为长江鲟早期发育阶段肠道菌群调控和健康养殖提供了参考依据.
To explore the mechanism of intestinal remodeling during the early development of Yangtze sturgeon(Acipenser dabryanus)under artificial breeding conditions,this study applied high-throughput sequencing technology to study the structural characteristics and correlation between the intestinal microbiota and biological feed,Tubificidae,in the early developmental stage of the fish.The results showed that Proteobacteria was the dominant phylum in the intestine of A.dabryanus before feeding,and Proteobacteria and Firmicutes were the main bacteria during the 10th to 40th day of feeding.At the genus level,during the period from not feeding to the 20th day of feeding,the intestinal microbiota was relatively singular,with Pandoraea occupying an absolute dominant position,accounting for more than 88%.On the 30th day of feeding,the intestinal microbiota of A.dabryanus underwent reorganization,with species richness and diversity reaching their peak.The dominant genera became Citrobacter,Lactococcus,Plesiomonas,Staphylococcus,Aeromonas,and Klebsiella.Procrustes analysis showed that,on the 40th day of feeding,the intestinal microbiota of A.dabryanus tended to be consistent with the microbial community composition of the biological feed,Tubificidae,showing a highly significant correlation(P<0.01).SourceTracker analysis verified that 18.97%of the A.dabryanus intestinal community on the 10th day of open feeding came from the biological feed,Tubificidae.On the 20th day of open feeding,it decreased to 0.15%,and on the 30th day of open feeding,it increased to 51.22%.On the 40th day of open feeding,it reached as high as 97.55%.This study indicated that when feeding A.dabryanus the biological feed,Tubificidae,a turning point in the early developmental stage of the intestinal microbiota occurs on the 30th day of feeding.At this time,the characteristics of the intestinal microbiota changed from high conservatism to good plasticity.Continuing feeding until the 40th day,the intestinal microbiota showed a high consistency with the biological feed microbial community.The results provided a reference for the regulation of intestinal microbiota and healthy cultivation of A.dabryanus in the early development.
田甜;张建明;朱欣;张德志;胡亚成
中国长江三峡集团有限公司中华鲟研究所,三峡工程鱼类资源保护湖北省重点实验室,湖北 宜昌 443100
水产学
长江鲟高通量测序早期发育肠道菌群生物饵料
Acipenser dabryanushigh-throughputearly developmentintestinal microbiotabiological feed
《中国水产科学》 2024 (005)
546-556 / 11
湖北省自然科学基金面上项目(2023AFB989).
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