中国水稻科学2024，Vol.38Issue(4)：375-385,11.DOI:10.16819/j.1001-7216.2024.230603
转录组和代谢组联合分析揭示稻曲病菌的致病因子
Combined Transcriptome and Metabolome Analyses Reveals the Pathogenic Factors of Ustilaginoidea virens
摘要
Abstract
[Objective]Rice false smut,caused by Ustilaginoidea virens,is a global fungal disease that severely impacts rice yield and quality.Understanding the molecular mechanisms behind U.virens pathogenicity could inspire novel control strategies and molecular breeding for disease resistance.[Method]Transcriptome and metabolome analyses were conducted on the initial symptomatic panicle nine days after inoculation(S)and uninoculated PXD25 mycelium(CK).The genome of strain UV-8b served as the reference for sequence alignment.Gene expression levels were quantified using fragments per kilobase per million fragments(FPKM),and differentially expressed genes(DEGs)were identified using|LOG2 fold change|≥ 1 and q-val ue≤0.05 criteria.Differentially accumulated metabolites(DAM)were identified with P≤0.05 and VIP≥1 thresholds.[Result]In the S vs.CK comparison,6078 DEGs were identified.GO enrichment analyses revealed 3708 terms,and KEGG pathway analysis identified 110 metabolic pathways.Ninety-one transcription factors(TFs)belonging to 23 TF families,such as bZIP and C2H2,were among the DEGs.Significant enrichment of mitophagy,secondary metabolism,and amino acid metabolism pathways was observed,with upregulation of DEGs in secondary metabolic pathways suggesting their crucial role in U.virens pathogenesis.Additionally,392 DAMs were identified,including alanine,tyrosine,histidine,methionine,cysteine,and fatty acids(linoleic acid,palmitic acid,lauric acid,myristic acid),indicating their association with U.virens pathogenicity.Combined transcriptome and metabolome analysis highlighted enrichment in amino acid metabolic pathways(e.g.,phenylalanine,tyrosine,cysteine,and methionine)and carbohydrate-related pathways(e.g.,starch and sucrose).[Conclusion]DEGs involved in mitophagy,secondary metabolism,and amino acid metabolism,along with DAMs like alanine,tyrosine,histidine,methionine,cysteine,and fatty acids,are closely linked to U.virens pathogenicity.关键词
稻曲病菌/转录组/代谢组/差异表达基因/差异代谢物Key words
Ustilaginoidea virens/transciptome/metabolome/differentially expressed genes/differential accumulated metabolites(DAM)引用本文复制引用
伏荣桃,陈诚,王剑,赵黎宇,陈雪娟,卢代华..转录组和代谢组联合分析揭示稻曲病菌的致病因子[J].中国水稻科学,2024,38(4):375-385,11.基金项目
四川省科技计划资助项目(2022YFYZ0002) （2022YFYZ0002）
四川省财政自主创新计划资助项目(2022ZZCX020) （2022ZZCX020）
四川省财政农作物"卡脖子"技术攻关计划资助项目(2021ZYGG-002). （2021ZYGG-002）
