中国生物防治学报2024，Vol.40Issue(3)：608-619,12.DOI:10.16409/j.cnki.2095-039x.2023.11.022
枯草芽胞杆菌GXHZ16抑制烟草疫霉病菌的表达谱分析
Expression Profiles of Bacillus subtilis GXHZ16 against Phytophthora nicotiana
摘要
Abstract
Tobacco black shank caused by Phytophthora nicotianae,is a soil-borne disease with wide distribution and serious damage in tobacco production.Bacillus subtilis(GXHZ16 strain)in our laboratory,which exhibited high antagonistic activity against P.nicotiana(inhibition rate:65.79%),was previously isolated and identified from the rhizosphere soil of healthy tobacco in Fuchuan County,Hezhou,Guangxi Province.To explore the possible inhibitory molecular mechanism of GXHZ16 strain against P.nicotiana,the mixed samples of B.subtilis GXHZ16 and P.nicotiana cultured for 36 h(treatment group)and 0 h(control group)were collected,respectively,and then the differentially expressed genes and related signaling pathways in B.subtilis GXHZ16(cultured for 36 h against P.nicotiana)were investigated by high-throughput transcriptome sequencing(RNA-Seq)and bioinformatics analysis.Our results showed that 1607 genes of B.subtilis GXHZ16 were differentially expressed after 36 h of confrontation culture,including 810 up-regulated genes and 797 down-regulated genes.These differentially expressed genes with up-regulation,including genes related to Flagellar assembly and Bacterial chemotaxis,as well as ten related genes associated with the synthesis of surfactin,fengycin and plipastatin,may be involved in the inhibition of B.subtilis against P.nicotiana.Furthermore,GO enrichment analysis showed that a total of 1343 differentially expressed genes have been annotated into different functional subclasses,and the cellular anatomical entity in cell component category was the one with the most differentially expressed genes.More importantly,KEGG enrichment analysis showed that 551 differentially expressed genes were classified into hundreds of pathways.The most differentially expressed genes were involved in the pathway of Microbial metabolism in diverse environments.Other major metabolic pathways included Flagellar assembly and Starch and sucrose metabolism.Moreover,Bacterial chemotaxis pathway and Monobactam biosynthesis pathway were highly enriched.In conclusion,gene expression profiles of B.subtilis(after confrontation culture with P.nicotiana)and related regulatory metabolic pathways were acquired based on transcriptomic analysis in this study,which will provide a basis for further elucidate the biocontrol mechanism of Bacillus to inhibit plant pathogenic fungi and improve the biocontrol efficiency of B.subtilis.关键词
枯草芽胞杆菌/烟草疫霉菌/转录组分析/差异表达基因Key words
Bacillus subtilis/Phytophthora nicotianae/transcriptomic analysis/differentially expressed genes分类
农业科技引用本文复制引用
胡亚杰,范啟铃,李群岭,周效峰,丁婷,汪章勋,吴峰..枯草芽胞杆菌GXHZ16抑制烟草疫霉病菌的表达谱分析[J].中国生物防治学报,2024,40(3):608-619,12.基金项目
广西特色烤烟专用功能肥料的研制与应用(2021450000340019) （2021450000340019）
