中国生物防治学报2024，Vol.40Issue(3)：620-629,10.DOI:10.16409/j.cnki.2095-039x.2024.02.023
摩氏假单胞菌环脂肽合成基因簇xtlABC的功能研究
Functional Study of Gene Cluster xtlABC Producing Cyclic Lipopeptide in Pseudomonas mosselii PtA1
摘要
Abstract
Previous studies have demonstrated that Pseudomonas mosselii PtA1 displays high levels antibacterial activities against various strains of Ralstonia solanacearum.Its genome encompasses a complete xtlABC gene cluster,which is responsible for the production of cyclic lipopeptide.In order to explore the function of xtlABC gene cluster in P.mosselii PtA1,we employed homologous recombination to create deletion mutants ΔxtlA,ΔxtlBC and ΔxtlABC,verified through Southern blot analysis,and assessed the phenotypic changes in growth rate,swimming motility,biofilm formation and antagonistic activity.The results revealed that compared with the wild-type strain PtA1,mutants ΔxtlA,ΔxtlBC and ΔxtlABC displayed a significant reduction in growth rate.The diameters of swimming rings were diminished by 12.6 mm,12.5 mm and 13.5 mm,respectively,the amount of biofilm formation decreased by 30.41%,29.16%and 30.70%,respectively,and the diameters of the antibacterial zone against R.solanacearum were also notably reduced.Pot experiments demonstrated that the control efficacy of treatment groups PtA1+GMI1000,ΔxtlA+GMI1000,ΔxtlBC+GMI1000 and ΔxtlABC+GMI1000 on tomato bacterial wilt were 44.93%,9.49%,10.46%and 7.66%4 days post-inoculation with R.solanacearum GMI1000,respectively.The control efficacies were 42.43%,19.56%,31.40%and 24.79%10 days post-inoculation,respectively.The treatment group PtA1+GMI1000 had the best control effect on tomato bacterial wilt,significantly different compared to the xtlABC mutant treatment groups.These findings underscore the impact of the xtlABC gene cluster on the growth and biocontrol-related characteristics of P.mosselii PtA1,and its secondary metabolite played an important role in inhibiting R.solanacearum.关键词
摩氏假单胞菌/环脂肽/基因簇xtlABC/青枯菌/抑菌作用Key words
Pseudomonas mosselii/cyclic lipopeptide/xtlABC/Ralstonia solanacearum/antibacterial activity分类
植物保护学引用本文复制引用
郑汉维,谢文松,牛世霖,张旭,卓涛,邱思鑫,范晓静..摩氏假单胞菌环脂肽合成基因簇xtlABC的功能研究[J].中国生物防治学报,2024,40(3):620-629,10.基金项目
国家自然科学基金(31801696) （31801696）
福建省属公益类科研院所基本科研专项(2022R1031008) （2022R1031008）
国家甘薯产业技术体系(CARS-10) （CARS-10）
福建农林大学科技创新专项基金(KFB23010) （KFB23010）
