中国生物防治学报2024，Vol.40Issue(3)：690-700,11.DOI:10.16409/j.cnki.2095-039x.2024.02.017
防御假单胞ZF509的分离鉴定及其对甜瓜细菌性果斑病的防治效果研究
Isolation and Identification of Pseudomonas ZF509 and Its Control Effect on Bacterial Fruit Blotch of Melon
摘要
Abstract
In order to obtain the biocontrol potential strain against bacterial fruit blotch(BFB),strain ZF509 isolated from potato rhizosphere soil in Heilongjiang Province was tested against Acidovorax citrulli.The stain ZF509 had biocontrol efficiency on BFB.Morphological and biochemical characterization tests were carried out on the stain ZF509 for identification.Phylogenetic analysis based on multilocus sequence revealed that stain ZF509 clustered with Pseudomonas protegens.Agar disk diffusion test showed that strain ZF509 had inhibitory effect on 4 pathogenic bacterial and 8 pathogenic fungi.Enzyme activity detection showed that strain ZF509 could dissolve phosphorus,secrete protease,synthesize biofilm and produce hydrocyanic acid.PCR amplification of genes of four bacteriostatic substances with specific primers showed that strain ZF509 had genes of producing 2-hydroxylated phenazine,2,4-DAPG,pyrrolnitrin and pyoluteori.Pot experiments showed that the disease index significantly reduced after inoculation with ZF509 and the control efficacy was up to 73.46%,which was superior to that of benziothiazolinone.The strain ZF509 has excellent biocontrol and practical potential against BFB.关键词
防御假单胞菌/果斑病/防治效果/抑菌物质Key words
Pseudomonas protegens/bacterial fruit blotch/biocontrol efficiency/antibacterial substances分类
农业科技引用本文复制引用
赵子璇,李俊辉,杜公福,石延霞,谢学文,柴阿丽,范腾飞,李宝聚,李磊..防御假单胞ZF509的分离鉴定及其对甜瓜细菌性果斑病的防治效果研究[J].中国生物防治学报,2024,40(3):690-700,11.基金项目
三亚崖州湾科技城专项(SCKJ-JYRC-2022-105) （SCKJ-JYRC-2022-105）
中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS) （CAAS-ASTIP-IVFCAAS）
农业农村部园艺作物生物学与种质创制重点实验室项目 （）
蔬菜生物育种全国重点实验室项目 （）
