中国生物防治学报2024，Vol.40Issue(3)：701-709,9.DOI:10.16409/j.cnki.2095-039x.2024.02.028
烟草赤星病拮抗菌群发酵条件优化及可湿性粉剂研制
Optimization of Fermentation Conditions for Antagonistic Bacteria of Tobacco Brown Spot and Development of Wettable Powder
摘要
Abstract
To develop an efficient biocontrol antimicrobial agent of tobacco brown spot,single factor test and orthogonal test design were used to optimize the fermentation process and wettable powder(WP)was developed,and its control effect was verified.The optimal fermentation medium for antagonistic bacteria was:glucose 10 g/L,tryptone 2.5 g/L,yeast powder 2.5 g/L,NaCl 10 g/L.The optimal fermentation conditions were as follows:pH 8.0,fermentation temperature 35℃,inoculation concentration 7%,125 mL medium in 250 mL flask.The optimal formulation of WP was silica(15%)as carrier,sodium lignosulfonate(1%)as dispersing agent,Tween 80(10%)as wetting agent,K2HPO4(2%)as stabilizer,carboxymethyl cellulose(0.1%)as protective agent.After 7 d and 14 d treatment in the field,the efficacy of WP against tobacco brown spot disease was 51.58%and 52.81%,respectively,indicating that the WP had certain application prospect in biocontrol.关键词
拮抗菌群/发酵条件优化/可湿性粉剂/烟草赤星病/防治效果Key words
antagonistic bacterium/optimization of fermentation condition/wettable powder/tobacco brown spot/control effect分类
农业科技引用本文复制引用
陈奕冰,刘索妮,伍绍龙,黄妤,唐前君,刘天波,向丹东,滕凯,肖志鹏,周向平,肖艳松,蔡海林,梅斯钇,李玲玲..烟草赤星病拮抗菌群发酵条件优化及可湿性粉剂研制[J].中国生物防治学报,2024,40(3):701-709,9.基金项目
湖南省重点领域研发计划项目(2023NK2019) （2023NK2019）
中国烟草总公司项目(110202101050(LS-10),110202201019(LS-03),110202201027(LS-11)) （110202101050(LS-10）
湖南省烟草公司衡阳市公司项目(HYYC2023KJ32) （HYYC2023KJ32）
中国博士后基金项目(2021M701177) （2021M701177）
湖南省教育厅重点项目(20A259) （20A259）
