中国生物防治学报2024，Vol.40Issue(3)：717-726,10.DOI:10.16409/j.cnki.2095-039x.2024.02.026
十字花科根肿病拮抗菌贝莱斯芽胞杆菌ZF72分离鉴定及生防效果研究
Identification and Biocontrol Effect of Bacillus velezensis ZF72 against Clubroot disease of Crucifers
摘要
Abstract
Clubroot disease of cruciferous plants caused by Plasmodiophora brassicae Woronin occurred severely and was difficult to control.In this study,a biocontrol agent ZF72 was screened by using dual-culture assay against Fusarium oxysporum and Phytophthora capsica.Control efficacy of ZF72 against clubroot disease in pot experiment was 74.6%.The strain ZF72 was identified as Bacillus velezensis based on morphological characteristics,physiological and biochemical analysis,as well as 16S rDNA,gyrB and atpD phylogenetic analysis.The fermentation of strain ZF72 was optimized by single factor test.An optimum medium composition was as followings:sorbitol 3 g/L,bran 8 g/L,CaCl2 1 g/L,and MgCl2·6H2O 3 g/L.The optimum fermentation conditions were as followings:temperature 28℃,pH 6.0,loading volume 50 mL/250 mL,rotating speed 180 r/min,and inoculation amount 1%(v/v).Treatment with strain ZF72 significantly reduced the incidence of clubroot disease of Chinese cabbage,with a control efficacy of 63.8%in field experiment,which provides new microbial resources for biological control of clubroot disease of crucifers.关键词
十字花科根肿病/贝莱斯芽孢杆菌/生物防治Key words
clubroot of crucifers/Bacillus velezensis/biocontrol分类
农业科技引用本文复制引用
林子涵,李宝聚,柴阿丽,张思雨,康华军,袁晓伟,李兴盛,石延霞,谢学文,李磊,范腾飞..十字花科根肿病拮抗菌贝莱斯芽胞杆菌ZF72分离鉴定及生防效果研究[J].中国生物防治学报,2024,40(3):717-726,10.基金项目
国家重点研发计划(2022YFD1401200) （2022YFD1401200）
中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS) （CAAS-ASTIP-IVFCAAS）
大宗蔬菜产业技术体系(CARS-25) （CARS-25）
