中国输血杂志2024，Vol.37Issue(7)：779-784,6.DOI:10.13303/j.cjbt.issn.1004-549x.2024.07.010
血小板微粒在糖尿病肾病患者中的表达及其与肾脏损伤的关系
Expression of platelet microparticles in patients with diabetic nephropathy and its relationship with renal damage
摘要
Abstract
Objective To investigate the expression levels of platelet microparticles(PMPs)in patients with diabetic nephropathy(DN)and their relationship with renal injury.Methods Thirty DN patients,30 T2DM patients without DN and 30 healthy controls were enrolled.Flow cytometry was used to detect the quantity and phenotype of PMPs,and ELISA was used to measure the levels of plasma renin,angiotensin Ⅱ(angiotoninⅡ,AngⅡ),vascular endothelial growth factor(vascular endothelial growth factor,VEGF)and transforming growth factor-β1(transforming growth factor-β1,TGF-β1).Kidney function was determined by blood biochemistry.Results The quantity of PMPs in the DN group was(1 564±346)particles/μL,which was significantly higher than that in the non-DN group(1 246±312)particles/μL and the control group(1 223±299)particles/μL(P＜0.05).The PMPs in the DN group mainly expressed CD62P and CD41a,accounting for(76.5±12.3)%.Moreover,the levels and activation of PMPs increased with the progression of DN.The quantity of PMPs was positively correlated with renin and other factors(r=0.56-0.62,P＜0.05),negatively correlated with the glo-merular filtration rate(GFR)(r=-0.64,P＜0.05),and positively correlated with the urinary albumin excretion rate(UAE)(r=0.66,P＜0.05).PMPs were also an independent influencing factor of UAE(β=12.34,P＜0.05).Conclusion PMPs expression is elevated in DN patients,which may be associated with renal injury,but it is insufficient to confirm its role in the pathogenesis of DN.关键词
血小板微粒/糖尿病肾病/肾脏损伤/血管紧张素系统激活/内皮功能障碍Key words
platelet microparticles(PMPs)/diabetic nephropathy(DN)/renal damage/activation of renin-angiotensin system/endothelial dysfunction分类
医药卫生引用本文复制引用
邵雯,杜彬,杜爱翠,任召祺..血小板微粒在糖尿病肾病患者中的表达及其与肾脏损伤的关系[J].中国输血杂志,2024,37(7):779-784,6.基金项目
国家卫生健康委基金项目(2021KYSHX02303) （2021KYSHX02303）
