中国输血杂志2024，Vol.37Issue(7)：820-826,7.DOI:10.13303/j.cjbt.issn.1004-549x.2024.07.018
4℃保存全血制备的去白细胞混合浓缩血小板体外质量评价
In vitro quality evaluation of leucocyte-reduced pooled platelet concentrates prepared from whole blood stored at 4℃
摘要
Abstract
Objective To investigate the feasibility of leucocyte-reduced pooled platelet concentrates from whole blood stored at 4℃,and provide theoretical basis for the components preparation.Methods The collected 400 mL ACD-B antico-agulant whole blood was randomly divided into two groups,stored at 4℃and room temperature.The buffy coat was prepared within 6 hours and store at 22℃until next day to prepare leucocyte-reduced pooled platelet concentrates.Platelet samples on day 1,3,5 and 7 were taken for the blood cell count and related parameter detection.The pH,glucose and lactic acid con-tent were determined to reflect the metabolic status,and the thromboelastography,platelet aggregation rate and PAC-1 and CD62P expression were determined to reflect the function and activation of platelets.The difference in platelets between two groups were analyzed.Results With the extension of storage time,the count of leucocyte-reduced pooled platelet concen-trates decreased gradually,but the platelets distribution width(PDW),mean platelet volume(MPV)and platelet-larger cell ratio(P-LCR)increased gradually in two groups,with no statistical significance(P＞0.05).The pH and glucose con-tents in two groups gradually decreased,but the lactic acid content gradually increased,with no significant difference(P＞0.05).The thrombelastogram showed MA value that reflecting platelet function has no significant change during the storage,and there was no significant difference between the two groups(P＞0.05).The aggregation rates decreased while the expres-sion of PAC-1 and CD62P increased gradually with the prolongation of preservation time,with no significant difference be-tween the two groups(P＞0.05).Conclusion There is no significant difference in platelet count,function and activation between whole blood stored at 4℃and at room temperature within 6 hours.Whole blood stored at 4℃within 6 hours can be considered as the raw material for leucocyte-reduced pooled platelet concentrates.关键词
去白细胞混合浓缩血小板/全血冷藏保存/血小板质量/血小板功能Key words
leucocyte-reduced pooled platelet concentrates/whole blood cold storage/platelet quality/platelet func-tion分类
医药卫生引用本文复制引用
安慧娟,单泓,刘铮,张姣姣,王姣杰,别立莉,李建斌,金新莉..4℃保存全血制备的去白细胞混合浓缩血小板体外质量评价[J].中国输血杂志,2024,37(7):820-826,7.基金项目
中国输血协会威高科研基金资助项目(CSBT-WG-2021-01) （CSBT-WG-2021-01）
河南省医学科技攻关计划联合共建项目(LHGJ20200252) （LHGJ20200252）
