中国动物传染病学报2024，Vol.32Issue(3)：72-78,7.
蜜蜂慢性麻痹病毒荧光定量RT-PCR检测方法的建立
Development of a Real-Time RT-PCR Method for Detection of Chronic Bee Paralysis Virus
摘要
Abstract
To establish a rapid diagnostic method for chronic bee paralysis virus(CBPV),a fluorogenic TaqMan real-time PCR assay was developed by designing specific primers and TaqMan probes according to the RNA dependent RNA polymerase(RdRp)gene sequence of CBPV in this study.The results showed that the real-time RT-PCR method had a good linear relation,and linear correlation coefficient R2 was 0.998.The sensitivity limit was 10 copies/μL and there was no cross-reaction with other Honey bee virus,which indicated that the method had good sensitivity and specificity.The coefficients of variation(CV)for intra-assay and inter-assay repeatability were less than 0.5％and 2％,respectively,showing that the method had good stability.The real-time RT-PCR method for CBPV developed in this study might be used for laboratory testing,epidemiological investigation and epidemic monitoring.关键词
蜜蜂慢性麻痹病毒/荧光定量RT-PCR/RNA依赖RNA聚合酶Key words
Chronic bee paralysis virus/real-time RT-PCR/RNA dependent RNA polymerase(RdRp)分类
农业科技引用本文复制引用
张体银,王武军,林素洁,张志灯,李宋钰,于师宇..蜜蜂慢性麻痹病毒荧光定量RT-PCR检测方法的建立[J].中国动物传染病学报,2024,32(3):72-78,7.基金项目
海关总署科研项目(2021HK161) （2021HK161）
福建省对外合作重点项目(202110029) （202110029）
福州海关科研项目(FK2020-21) （FK2020-21）
