中国动物传染病学报2024，Vol.32Issue(3)：173-177,5.
赛鸽圆环病毒天津株全基因组的扩增和序列分析
Amplification and Characterization of a Circovirus from Racing Pigeons in Tianjin
摘要
Abstract
In this study,a Pigeon circovirus(PiCV)was detected from a diarrheic racing pigeon in Tianjin,by using PCR method.Sequence analysis of this PiCV strain revealed a genome size of 2032 bp,consisting of 5 open reading frames(ORFs)and 2 intergenic regions located at the 5'and 3'ends.Analysis of the pairwise sequences showed that this Tianjin PiCV strain shared 81.5％-97.7％genomic identity with other PiCVs from China,Australia,Japan,Poland,Belgium and Brazil.Phylogenetic analysis was performed for the full genomes of 53 references and this Tianjin strain by neighbor-joining method.As a result,these 54 PiCV strains were divided into 6 clades(A,B,C,D,E and F).The Tianjin strain was located in the clade B and was closely related to the strains from Belgium(DQ915957 and DQ915958)and China(KX108826,KX108805,KX108825,KJ704802 and KJ704806).Recombination analyses were carried out with Simplot and found that Rep ORFs contained several recombination hotspots,suggesting genetic diversity of PiCVs in China.关键词
鸽圆环病毒/序列分析/重组分析Key words
Pigeon circovirus/phylogenetic analysis/recombination分类
农业科技引用本文复制引用
王志悦,李欣蕊,孙嘉咛,王许刚,王瑞,李春红,徐明举,张瑞华,徐彤..赛鸽圆环病毒天津株全基因组的扩增和序列分析[J].中国动物传染病学报,2024,32(3):173-177,5.基金项目
河北省科技厅重点研发计划(20326624D) （20326624D）
河北省高等学校科学技术研究项目(ZD2020314) （ZD2020314）
河北省高层次人才资助项目(A202003006) （A202003006）
河北省第二期现代农业产业技术体系蛋肉鸡创新团队建设专项经费(HBCT2018150207) （HBCT2018150207）
河北北方学院校级科研项目(YB2020006,XT2021008) （YB2020006,XT2021008）
