Viperin通过胆固醇影响禽传染性支气管炎病毒复制OA北大核心

Viperin is an interferon-inducible factor with broad antiviral activity.To investigate whether Viperin exerts its anti-avian infectious bronchitis virus(IBV)effects through cholesterol,this study analyzed the impact of cholesterol on IBV replication by depleting or adding cellular membrane cholesterol.The study also evaluated the effects of overexpressing and silencing Viperin on cellular cholesterol content and IBV replication and examined the relationship among Viperin,cholesterol content,and IBV replication.The results showed that,48 hours after IBV infection,compared with the IBV-infected group,IBV replication was significantly inhibited after depleting cellular membrane cholesterol with 5.0 and 10.0 mmol/L methyl-beta-cyclodextrin(MβCD)(P＜0.001),while adding 5.0 mg/mL exogenous cholesterol significantly promoted IBV replication(P＜0.001).Depleting cellular membrane cholesterol followed by adding exogenous cholesterol restored IBV replication to near-normal levels(P＞0.05).At 24 and 48 hours post IBV infection,overexpressing Viperin significantly reduced cellular cholesterol content(P＜0.001)and significantly inhibited IBV replication(P＜0.001),compared with the IBV-infected group.At 6 and 48 hours post IBV infection,silencing Viperin significantly increased cellular cholesterol content(P＜0.05 or P＜0.001)comparing with the IBV-infected group.At 24 hours post IBV infection,compared with the IBV-infected group,silencing Viperin significantly promoted IBV replication(P＜0.001),while overexpressing Viperin followed by adding exogenous cholesterol at 48 hours post IBV infection restored IBV replication(P＞0.05).The results indicate that the cholesterol level in the cellular membrane is closely related to IBV replication,and Viperin can inhibit IBV replication by regulating cellular cholesterol.This study provides a theoretical basis and new insights for identifying new antiviral targets against IBV.