番茄红素对限制性应激雄鼠生长性能和肠道抗氧化功能的影响OA北大核心

To investigate the effects of lycopene(LYC)on growth performance and intestinal antioxidant function in male mice under restraint stress,80 male mice were randomly divided into four groups:Control(CON)group,Restraint stress(RS)group,LYC[10 mg/(kg·bw)]+restraint stress(LRS)group,and Corn oil+restraint stress(CRS)group.The LRS and CRS groups were gavaged with LYC and corn oil(0.5 mL/mouse)respectively,2 hours before restraint stress treatment.The duration of restraint stress was 5 hours per day,continuously for 14 days.Daily records of body weight and feed intake before and after stress were kept.At the end of the experiment,tissues from the duodenum,jejunum,and ileum were collected.ELISA kits were used to detect the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),total antioxidant capacity(T-AOC),and the content of malondialdehyde(MDA)in the tissues.Western blot analysis was performed to detect the protein expression levels of phosphorylated protein kinase B/protein kinase B(p-AKT/AKT),nuclear factor erythroid 2-related factor(Nrf2),and heme oxygenase 1(HO-1).The results showed that compared with the RS group,the weight loss of male mice before and after stress in the LRS group was significantly reduced(P＜0.05),and the average daily feed intake showed an increasing trend(P＞0.05).The activities of SOD,GSH-PX,and T-AOC in the duodenum,jejunum,and ileum tissues were significantly increased(P＜0.05),while the MDA content was significantly decreased(P＜0.05).The p-AKT/AKT level in the ileum was significantly increased(P＜0.05),with an increasing trend in the duodenum and jejunum(P＞0.05).The relative expression levels of Nrf2 protein in the duodenum and jejunum were significantly increased(P＜0.05),with an increasing trend in the ileum(P＞0.05).The relative expression levels of HO-1 protein in the duodenum,jejunum,and ileum tissues were significantly increased(P＜0.05).The CRS group showed no significant differences in the above indicators compared to the RS group(P＞0.05),indicating that corn oil,serving only as a solvent,does not have a mitigating effect.These results indicate that lycopene can alleviate the adverse effects of restraint stress on the growth performance of male mice and effectively enhance the local antioxidant level in the intestines.The mechanism may be related to the activation of the AKT-Nrf2/HO-1 pathway.