生鲜乳中海氏肠球菌的分离鉴定OA北大核心

To investigate the presence of Enterococcus contamination in raw milk,seven raw milk samples were aseptically collected from a dairy farm in Shihezi.The bacterial isolates were identified through bacterial isolation and purification,biochemical identification,and sequence analysis of the 16S rRNA and RNA polymerase α subunit(rpoA)genes of Enterococcus.The antibiotic sensitivity and pathogenicity of the isolates were determined using the disk diffusion method and mouse pathogenicity test,respectively.The results showed that one bacterial strain was isolated from the raw milk.Its cultural and biochemical characteristics were similar to Enterococcus.The 16S rRNA gene sequencing indicated that the strain was Enterococcus,and the rpoA gene sequencing confirmed its molecular characteristics matched those of Enterococcus hirae.Antibiotic sensitivity tests revealed that the isolated Enterococcus hirae exhibited resistance to aminoglycosides,lincosamides,sulfonamides,and polypeptides,but was sensitive to varying degrees to tetracyclines,β-lactams,and some quinolones.Mouse pathogenicity tests indicated that the strain had a certain level of pathogenicity.This study,based on the isolation,identification,antibiotic resistance,and pathogenicity analysis of Enterococcus hirae in raw milk,preliminarily indicates the risk of Enterococcus contamination in the dairy farm's milk.The isolated strain poses certain antibiotic-resistant and pathogenic risks,emphasizing the need for enhanced monitoring during routine production and processing.