中国现代医学杂志2024，Vol.34Issue(13)：28-40,13.DOI:10.3969/j.issn.1005-8982.2024.13.005
LncRNA ZEB2-AS1联合亲本基因ZEB2在卵巢癌SKOV3细胞中的作用及其机制研究
Functional study of LncRNA ZEB2-AS1 and parental gene ZEB2 in promoting epithelial-mesenchymal transition in ovarian cancer SKOV3 cells
摘要
Abstract
Objective To explore the function and potential mechanism of LncRNA ZEB2-AS1 combined with its parental gene ZEB2 in ovarian cancer SKOV3 cells.Methods In situ hybridization and immunohistochemistry were used to detect the expression of ZEB2-AS1 and ZEB2 in ovarian cancer tissues.SKOV3 cell models with ZEB2-AS1 overexpression and silencing were established.Cell proliferation was measured using the CCK-8 assay.Flow cytometry was used to detect cell apoptosis and cycle.Epithelial-mesenchymal transition(EMT)-related protein expression was assessed through immunofluorescence and Western blotting.Dual-luciferase gene reporter assays were performed to confirm the binding relationship between ZEB2-AS1,ZEB2,and microRNA-145(miR-145).Results ZEB2-AS1 and ZEB2 were expressed in the cytoplasm and nucleus of ovarian cancer tissues.Compared with SKOV3 cells,ZEB2-AS1 overexpressing SKOV3 cells showed increased proliferation(P＜0.05),reduced apoptosis(P＜0.05),enhanced invasion and migration(P＜0.05),increased S-phase cells(P＜0.05),and decreased G,phase cells(P＜0.05).EMT-related protein expression showed a decrease in E-cadherin(P＜0.05)and an increase in N-cadherin and Vimentin(P＜0.05).Conversely,ZEB2-AS1 silenced SKOV3 cells exhibited opposite trends.Dual-luciferase reporter assay confirmed the binding relationship between ZEB2-AS1 and miR-145,as well as between ZEB2-AS1 and ZEB2 mRNA.Conclusions LncRNA ZEB2-AS1 combined with its parental gene ZEB2 promotes EMT in ovarian cancer SKOV3 cells.关键词
卵巢癌/LncRNA ZEB2-AS1/ZEB2/上皮-间充质相互作用Key words
ovarian cancer/LncRNA ZEB2-AS1/ZEB2/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
董辉,王晶,杨丹,丁永慧..LncRNA ZEB2-AS1联合亲本基因ZEB2在卵巢癌SKOV3细胞中的作用及其机制研究[J].中国现代医学杂志,2024,34(13):28-40,13.基金项目
宁夏自然科学基金(NO:2023AAC03601) （NO:2023AAC03601）
宁夏回族自治区重点研发计划(NO:2019BEH03005) （NO:2019BEH03005）
