中国现代医学杂志2024，Vol.34Issue(13)：41-48,8.DOI:10.3969/j.issn.1005-8982.2024.13.006
基于MAPK/ERK通路探讨茯苓多糖对卵巢早衰模型大鼠的作用及其分子机制
Study on the effect and molecular mechanism of Poria cocos polysaccharide on premature ovarian failure model rats based on MAPK/ERK pathway
摘要
Abstract
Objective To explore the effect and molecular mechanism of Poria cocos polysaccharide on premature ovarian failure(POF)model rats based on mitogen-activated protein kinase(MAPK)/extracellular regulated protein kinase(ERK)pathway.Methods The POF model was induced by intraperitoneal injection of cyclophosphamide in SD female rats,and was randomly divided into five groups:model group,low dose poria cocos polysaccharide(100 mg/kg)group,high dose poria cocos polysaccharide(200 mg/kg)group,anisomycin(MAPK activator,10 mg/kg)group,high dose poria cocos polysaccharide+anisomycin group,with 10 rats in each group,another 10 SD rats were injected intraperitoneally with the same dose of normal saline as the control group,the uterus index and ovary index of rats in each group were measured after treatment with Poria cocos polysaccharide and anisomycin;the levels of serum sex hormones[estradiol(E2),luteinizing hormone(LH)and follicle-stimulating hormone(FSH)]were measured by enzyme-linked immunosorbent assay(ELISA)kit;the ovarian morphology of rats in each group was observed by HE staining;the levels of tumor necrosis factor-α(TNF-α),interleukin-4(IL-4)and IL-18 in serum and ovarian tissue of rats in each group were measured by ELISA kit;the expression of MAPK/ERK pathway related protein in ovarian tissue of rats in each group was detected by Western blotting.Results Compared with the control group,the interstitial structure of the ovary in the model group had some degree of hemorrhage,fibrosis damage,and follicular dysplasia,the serum FSH and LH levels,serum and ovarian tissue TNF-α,IL-4,IL-18 levels,ovarian tissue p-p38 MAPK/p38 MAPK and p-ERK1/2/ERK1/2 were increased(P＜0.05),the uterine index,ovarian index and E2 level were decreased(P＜0.05).Compared with the model group,the ovarian morphology damage and follicular dysplasia of rats in the low dose group and the high dose Poria cocos polysaccharide group were alleviated,the serum FSH and LH levels,serum and ovarian tissue TNF-α,IL-4,IL-18 levels,ovarian tissue p-p38 MAPK/p38 MAPK and p-ERK1/2/ERK1/2 were all decreased(P＜0.05),uterine index,ovarian index and E2 level were increased(P＜0.05),and the effect of pachyman high dose group was better(P＜0.05);In the anisomycin group,the ovarian morphology damage and follicular dysplasia were aggravated,the levels of serum FSH and LH,serum and ovarian tissue TNF-α,IL-4,IL-18,and ovarian tissue p-p38 MAPK/p38 MAPK and p-ERK1/2/ERK1/2 were increased(P＜0.05),uterine index,ovarian index and E2 level decreased(P＜0.05).The high dose of pachyman+anisomycin group reversed the effect of pachyman,showing an opposite trend(P＜0.05).Conclusion Poria cocos polysaccharide can alleviate the inflammation of POF rats by inhibiting the activation of MAPK/ERK pathway,thereby improving the ovarian function and sex hormone secretion,and alleviating the symptoms of premature ovarian failure in rats.关键词
卵巢早衰/丝裂原活化蛋白激酶/细胞外调节蛋白激酶/茯苓多糖/分子机制Key words
premature ovarian failure/mitogen-activated protein kinase/extracellular regulated protein kinases/poria cocos polysaccharide/molecular mechanism分类
医药卫生引用本文复制引用
张静,高冬冬,谷灿灿,胡国华..基于MAPK/ERK通路探讨茯苓多糖对卵巢早衰模型大鼠的作用及其分子机制[J].中国现代医学杂志,2024,34(13):41-48,8.基金项目
上海市2020年度"科技创新行动计划"医学创新研究专项项目(No:20Z21900400) （No:20Z21900400）
