中国现代医学杂志2024，Vol.34Issue(13)：49-56,8.DOI:10.3969/j.issn.1005-8982.2024.13.007
4种程序性细胞死亡方式在轻型颅脑损伤中的发展探究
Exploration of development of four programmed cell death modes in mild traumatic brain injury
摘要
Abstract
Objective To investigate the occurrence and development of four programmed cell death modes(apoptosis,autophagy,pyroptosis,and ferroptosis)in mild traumatic brain injury with time.Methods 49 SPF Wistar rats were used to replicate the rat model with mild traumatic brain injury.7 were blank group and 42 were model group.Samples were collected at day 1,day 3,day 7,day 14,day 21 and day 28 after the successful replication of the model.The microstructure of rat hippocampal neurons was observed by hematoxylin-eosin(HE),Nissl's staining,uranium lead double staining,respectively.Western blotting was used to detect the expressions of Bcl-2,LC3B,FTH and caspase 1 proteins in the hippocampus of each group of rats.Results Optical microscopy showed that the neurons in the model group were crumpled.The results of electron microscopy and Western blotting showed that compared with the blank control group,the neurons in the model group were slightly contracted at day 1,and the relative expression of Bcl-2 protein was higher(P＜0.05).In the model group,the cell membrane was damaged in many places and the organelles were swollen at day 3 and day 7.The expression of caspase1 protein in the model group reached the highest level at day 3(P＜0.05).On the 14th day of the model group,the mitochondrial ridge of neurons was broken more locally,and the relative expression of FTH protein was also higher(P＜0.05).The relative expression of FTH protein in the model group was still increased on day 28(P＜0.05);There was a small amount of primary lysosome or autophagic lysosome in the cells of the model group on day 28,but the relative expression level of LC3B protein in the model group on day 28 was lower than that on day 21(P＜0.05).Conclusion After mild traumatic brain injury,more neurons chose apoptosis at the first time,pyroptosis followed,ferroptosis occurred later,and autophagy continued.关键词
神经元/轻型颅脑损伤/凋亡/自噬/铁死亡/焦亡Key words
neuron/mild traumatic brain injury model/apoptosis/autophagy/ferroptosis/pyroptosis分类
医药卫生引用本文复制引用
王恬恬,赵乐,冯忆,魏楠楠,刘骐肇,高丽,王永辉..4种程序性细胞死亡方式在轻型颅脑损伤中的发展探究[J].中国现代医学杂志,2024,34(13):49-56,8.基金项目
国家自然科学基金(No:82104972) （No:82104972）
山西省卫生健康委科研项目(No:2021041) （No:2021041）
山西省中医药管理局科研课题计划(No:2022ZYYC267) （No:2022ZYYC267）
山西中医药大学博士科研启动基金资助(No:2022BK019) （No:2022BK019）
山西中医药大学科技创新能力培育计划(No:2023PY-YS-03) （No:2023PY-YS-03）
