中国畜牧兽医2024，Vol.51Issue(7)：2739-2750,12.DOI:10.16431/j.cnki.1671-7236.2024.07.002
广西麻鸡卵巢组织中差异lncRNA筛选及ceRNA网络构建
Screening of Differential lncRNAs and Construction of ceRNA Network in Ovary Tissue of Guangxi Ma Chicken
摘要
Abstract
[Objective]This study was aimed to screen differentially expressed long non-coding RNA(lncRNA),messenger RNA(mRNA)and microRNA(miRNA)of ovary in Guangxi Ma chicken,and further select lncRNA related to egg production traits,providing theoretical support for the study of lncRNA regulation of egg production traits in chickens.[Method]Based on the egg production of Guangxi Ma chickens from start of production to 41 weeks of age,three high-yielding and three low yielding Guangxi Ma chickens were selected,and their ovarian tissues were used as materials.RNA sequencing technology was used to sequence them.Differentially expressed lncRNA,mRNA,and miRNA were screened from the sequencing data using edgER and DESeq2 software.the target genes of miRNA and lncRNA were predicted by miRanda software and cis-acting of lncRNA,respectively.GO function and KEGG pathway enrichment analysis were performed on the target genes of differentially expressed lncRNA,and a ceRNA network diagram was constructed using Cytoscape(v 3.8.2)software The transcriptome sequencing results were verified by Real-time quantitative PCR.[Result]RNA sequencing technology was used to obtain 438 differentially expressed lncRNAs,18 differentially expressed miRNAs,and 301 differentially expressed mRNAs in the ovaries of Guangxi Ma chicken.GO function and KEGG pathway enrichment analysis of the target genes of the differentially expressed lncRNAs showed significant enrichment in bioprocesses related to hormone secretion regulation,mesoderm formation,mesoderm development,and mesoderm morphogenesis related to egg production,as well as signaling pathways such as MARK,biosynthesis of glycosaminoglycans,oocyte meiosis,progesterone-mediated oocyte maturation,and gonadotropin-releasing hormone.Through GO function and KEGG pathway enrichment analysis,six lncRNAs related to egg production traits were obtained,including TCONS_00091814,TCONS_00051866,XR_006939520.1,XR_005839807.1,TCONS_00088588 and TCONS_00037324.A ceRNA network map containing 9 miRNAs with 60 lncRNAs and 3 mRNAs was successfully constructed based on the sequencing results,and all the genes in the network were differentially expressed in the low and high yield groups.Real-time quantitative PCR results of the selected differentially expressed genes were consistent with the gene expression levels from transcriptome sequencing,indicating that the transcriptome sequencing results were reliable.[Conclusion]A series of differentially expressed lncRNAs were screened from ovary in Guangxi Ma chicken using RNA sequencing technology,and six lncRNAs possibly associated with egg production traits were identified,providing theoretical support for further enhancing egg production performance in chickens.关键词
广西麻鸡/lncRNA/差异表达/产蛋性状/ceRNAKey words
Guangxi Ma chicken/lncRNA/differential expression/egg production traits/ceRNA分类
农业科技引用本文复制引用
张珂,张敏,李添宝,张杰,卢晟盛,江明生..广西麻鸡卵巢组织中差异lncRNA筛选及ceRNA网络构建[J].中国畜牧兽医,2024,51(7):2739-2750,12.基金项目
广西南宁市良庆区科学研究与技术开发项目(202108) （202108）
