中国畜牧兽医2024，Vol.51Issue(7)：2943-2952,10.DOI:10.16431/j.cnki.1671-7236.2024.07.020
千金藤素对牛体外卵母细胞和早期胚胎抗凋亡能力的影响
Effect of Cepharanthine on Anti-apoptotic Ability of Bovine Oocytes and Early Embryos in vitro
摘要
Abstract
[Objective]This study was aimed to explore the effect of cepharanthine(Ceph)on the antiapoptotic capacity of bovine oocytes and early embryos,so as to provide reference for improving the efficiency of bovine embryo production in vitro and new data for the pharmacological action of Ceph in anticancer process.[Method]Bovine oocytes were cultured in in vitro mature medium containing different concentrations(0,10,100,500 and 1 000 μmol/L)of Ceph to observe the oocyte maturation rate and early embryo development after fertilization,the optimal concentration of Ceph was determined.The cathepsin B(CB)activity,the cell autophagy-related protein(LC3)level,the mRNA expression of apoptosis-related genes Survivin and Caspase-3,and the apoptosis rate of blastocysts in bovine oocyte were detected.[Result]The maturation rate of bovine oocytes and the division rate of early embryo treated with 500 μmol/L Ceph were significantly higher than that of other groups(P＜0.05).The addition of Ceph significantly reduced the CB activity and LC3 level in bovine oocytes(P＜0.05),significantly increased the mRNA expression of Survivin gene(P＜0.05),and significantly reduced the mRNA expression of Caspase-3 gene and the apoptosis rate of blastocysts(P＜0.05).[Conclusion]The addition of Ceph during in vitro embryo production could regulate the expression of apoptosis-related genes Survivin and Caspase-3 to affect the levels of CB and LC3,and enhance the anti-apoptotic capacity of bovine oocytes and in vitro embryo production efficiency.关键词
千金藤素/牛/卵母细胞/早期胚胎/抗凋亡Key words
cepharanthine/cattle/oocyte/early embryo/anti-apoptosis分类
农业科技引用本文复制引用
郑湘民,王玉琪,徐冰洁,孙朝阳,高青山,方南洙,金庆国..千金藤素对牛体外卵母细胞和早期胚胎抗凋亡能力的影响[J].中国畜牧兽医,2024,51(7):2943-2952,10.基金项目
国家自然科学基金"氧化应激诱导的母型-合子型时期哺乳动物胚胎发育阻滞机理的研究"(32060756) （32060756）
高等学校学科创新引智计划项目(D20034) （D20034）
