中国畜牧兽医2024，Vol.51Issue(7)：3030-3040,11.DOI:10.16431/j.cnki.1671-7236.2024.07.028
一株孔雀源奇异变形杆菌全基因组测序及毒力与耐药性分析
Whole Genome Sequencing and Virulence and Drug Resistance Analysis of a Strain of Proteus mirabilis Derived from Peacock
摘要
Abstract
[Objective]The aim of this study was to investigate the virulence,drug resistance and genomic characteristics of Proteus mirabilis PM19 isolated from blue peacock.[Method]Animal experiments,drug sensitivity tests and PCR methods were used to detect the virulence,drug resistance and virulence genes of the isolated bacteria.The Illumina NovaSeq6000 platform was used to sequence the genome frame diagram,and the functional genes,pathogenic host interaction protein,carbohydrate active enzyme,drug resistance gene and virulence factor prediction were carried out by NR,KEGG,Swiss-Prot,COG,PHI-base,CAZy,CARD and VFDB databases.[Result]The isolate PM19 was a multidrug-resistant bacterium and was resistant to 14 antimicrobial agents including ampicillin,amoxicillin/clavulanate potassium,ceftriaxone,cefotaxime,amikacin,gentamicin,kanamycin,ciprofloxacin,tetracycline,doxycycline,sulfadiazine sodium,tilmicosin,florfenicol and chloramphenicol.The isolated bacteria had strong virulence,and the median lethal dose to mice was 6.19 ×106 CFU.Eight virulence genes including FliL,zapA,rsmA,hmpA,mrpA,atfA,pmfA,ureC were detected in the strain.The whole genome sequencing analysis showed that the genome size of the strain was about 3.98 Mb,the GC content was 38.94％,and 3 715 coding genes were predicted.158 protein genes related to pathogen-host interaction were annotated by PHI-base database.101 carbohydrate active enzyme genes were annotated by CAZy database.92 drug resistance genes and 8 virulence-related genes were annotated by CARD database and VFDB database,respectively.[Conclusion]The Proteus mirabilis strain PM19 was virulent and multidrug-resistant,carrying a large number of pathogenic host interaction protein genes,carbohydrate active enzyme genes,drug resistance genes and virulence genes.关键词
奇异变形杆菌/多重耐药性/毒力/全基因组测序Key words
Proteus mirabilis/multi-drug resistant/virulence/whole genome sequencing分类
农业科技引用本文复制引用
焦凤超,雷震,李迎晓,武娴,曲哲会..一株孔雀源奇异变形杆菌全基因组测序及毒力与耐药性分析[J].中国畜牧兽医,2024,51(7):3030-3040,11.基金项目
信阳农林学院科技创新团队项目(XNKJTD-014) （XNKJTD-014）
信阳农林学院青年基金项目(QN2022005) （QN2022005）
河南省科技攻关项目(182102110318) （182102110318）
