中国烟草学报2024，Vol.30Issue(4)：74-83,10.DOI:10.16472/j.chinatobacco.2023.T0152
普通烟草HSP20基因家族成员鉴定及表达分析
Identification and expression analysis of HSP20 gene family members in common tobacco
摘要
Abstract
[Background]Heat Shock Protein 20(HSP20)is an important protein family that plays a critical role in plant growth,development,and stress resistance.Currently,there is limited comprehensive research on HSP20 genes in tobacco.[Methods]Using bioinformatics analysis tools and the Solanaceae database,this study identified members of the HSP20 family in common tobacco and conducted related analyses.[Results]Fifty-eight HSP20 family genes were identified in tobacco,and evolutionary analysis divided them into 13 subfamilies,distributed across 13 chromosomes.Collinearity analysis indicated that there were 5 pairs of genes with chromosome segment duplications in tobacco.Cis-acting element analysis showed that upstream of NtHSP20 members contained a variety of cis-acting elements related to growth and stress response.The HSP20 family genes exhibited significant tissue-specific expression,with higher gene expression quantity and level in roots and stems,and lower in flowers.qRT-PCR analysis indicated that some members of the NtHSP20 family have a specific response to drought stress,with significant differences in gene expression levels at different stress stages.[Conclusion]This study lays the foundation for further research into the mechanisms of growth,development,and stress resistance in the tobacco HSP20 family.关键词
烟草/NtHSP20/进化分析/抗逆胁迫/表达分析Key words
tobacco/NtHSP20/phylogenetic analysis/stress resistance/expression analysis引用本文复制引用
赵泽玉,管恩森,孙刚,王大海,王朔,李志远,杜甫,李子玮,王琰琰,王荣浩,施友志,孙晋浩,田震,赵清海..普通烟草HSP20基因家族成员鉴定及表达分析[J].中国烟草学报,2024,30(4):74-83,10.基金项目
中国烟草总公司重大科技项目"基于工业导向的云烟87多性状聚合育种研究"(110202201014(JY-14)) （110202201014(JY-14）
中央级公益性科研院所基本科研业务费专项(1610232023013) （1610232023013）
江苏中烟科技项目"中烟特香301新品种烟叶品质保障技术及应用研究"(H202110) （H202110）
