中国烟草学报2024，Vol.30Issue(4)：84-94,11.DOI:10.16472/j.chinatobacco.2023.101
一株具有多糖降解酶活性的真菌菌株的筛选及其在烟叶品质提升中的应用研究
Screening of a fungus with polysaccharide-degrading enzyme activity and studies of its application in improving the quality of tobacco leaves
摘要
Abstract
[Background]To screen microbial strains that are effective in improving the quality of tobacco leaves.[Methods]A fungal strainwith strong capacity to secret polysaccharide-degrading enzymes was screened fromtobacco leaves that had been aged for two years,and subjected for species identification,fermentation condition optimization,and application in the treatment of tobacco leaves.[Results](1)Five fungi were isolated from tobacco leaves,and through the comparative analysis of their enzyme activities,one strain S1 with high polysaccharide degrading enzyme activities was screened.Morphological and molecular biological analyses showedthat the strainis Aspergillus aculeatus.(2)The fermentation condition of strain S1 was optimized,and it was found that the highest enzyme production was achieved at pH 6,30℃,and inoculum of 10%.Under this condition,the activities of cellulase,amylase,arabiofuranosidase,pectinase,xylanase and β-glucosidase reached 0.36 U/mL,8.48 U/mL,0.25 U/mL,3.71 U/mL,17.98 U/mL and 3.19 U/mL,respectively.(3)Tobacco samples treated with the fermentation broth of S1 had significantly increased contents of soluble sugars as well as aroma constituents such as various acids,ketones,aldehydes,aromatics,and alcohols,while the contents of nicotine and polysaccharide significantly decreased.[Conclusion]In conclusion,a A.aculeatus strainwith polysaccharide-degrading enzyme activities was isolated,and it has great development potential in improving the quality of tobacco leaves.关键词
棘孢曲霉/真菌/烟叶提质/生物酶Key words
Aspergillus aculeatus/fungi/quality improvement of tobacco leaves/biological enzymes引用本文复制引用
张怡帆,孙帅帅,胡苏林,杜文杰,郝贤伟,任志广,刘永新,郑力文,高丽伟,张鹏..一株具有多糖降解酶活性的真菌菌株的筛选及其在烟叶品质提升中的应用研究[J].中国烟草学报,2024,30(4):84-94,11.基金项目
山东中烟工业有限责任公司科技项目"提升山东烟叶可用性的定制复合酶制剂研究"(2022370000340061) （2022370000340061）
中国烟草总公司重点研发项目"基于分子设计的烟草专用生物酶的定向改造及应用研究"(110202102018) （110202102018）
