中国烟草学报2024，Vol.30Issue(4)：95-104,10.DOI:10.16472/j.chinatobacco.2023.T0178
NtJAZ9基因参与调控烟草腺毛发生的功能研究
Functional Study on the Role of NtJAZ9 Gene in Regulating the Formation of Tobacco Glandular Trichomes
摘要
Abstract
[Purpose]This study aims to explore the molecular mechanism of jasmonic acid in regulating tobacco trichomes glandular trichomes occurrence.[Methods]Homologous cloning,bioinformatics analysis and gene function studies of NtJAZ9,a key repressor of jasmonic acid signaling,were conducted[Results](1)NtJAZ9 has two homologous sequences,NtJAZ9-1 and NtJAZ9-2,with coding regions of 1008 bp and 996 bp,respectively.(2)Subcellular localization results indicate that the NtJAZ9 protein is localized in the nucleus.In various tissues,the relative expression of NtJAZ9-1 is higher than that of NtJAZ9-2,with the highest expression of NtJAZ9-1 in stem epidermis and the highest expression of NtJAZ9-2 in leaves.(3)Overexpression of NtJAZ9 results in reduced glandular trichome density on leaves,while knockout of the NtJAZ9 gene significantly increases glandular trichome density;the difference in protective trichome density is not significant.Methyl jasmonate(MeJA)can significantly promote the occurrence of long-stalk glandular trichomes.After spraying,the density of long-stalk glandular trichomes increased by 1.76 times in overexpressed strains and by 0.36 times in knockout strains.[Conclusion]The NtJAZ9 gene is involved in the regulation of occurrence of tobacco glandular trichomes and affects the regulatory role of MeJA signals on the formation of tobacco glandular trichomes to a certain extent.关键词
烟草/NtJAZ9/过表达/基因编辑/腺毛密度/MeJAKey words
tobacco/NtJAZ9/overexpression/gene editing/glandular trichome density/MeJA引用本文复制引用
朱文奇,张新要,裴文毅,季盈彤,董千锋,廖子鑫,闫筱筱,崔红..NtJAZ9基因参与调控烟草腺毛发生的功能研究[J].中国烟草学报,2024,30(4):95-104,10.基金项目
国家自然科学基金青年基金"中国野生毛葡萄VqJAZ4抗白粉病功能及调控机制研究"(32102361) （32102361）
湖南中烟工业有限责任公司合作项目"多腺毛、高烟碱K326种质创制和育种应用研究—多腺毛高烟碱K326材料的创制和分析"(202143000834089) （202143000834089）
