中国药房2024，Vol.35Issue(14)：1673-1682,10.DOI:10.6039/j.issn.1001-0408.2024.14.01
纳米孔测序在病原微生物检测中的应用专家共识
Expert consensuses on the application of nanopore sequencing technology in the detection of pathogenic microorganisms
摘要
Abstract
OBJECTIVE To improve the diagnosis and treatment level of critically ill infectious diseases,standardize the clinical application of nanopore sequencing and promote the sound development of the technology.METHODS Division of Therapeutic Drug Monitoring of Chinese Pharmacological Society and Expert Committee of Precision Medicine for Clinical Treatment of Guangdong Pharmaceutical Association initiated and organized multidisciplinary experts to discuss and determine the consensus writing outline by using the nominal group method,forming a preliminary consensus draft;expert consultation was performed by using Delphi method,and then experts'opinions were analyzed and revised to form consensus.RESULTS&CONCLUSIONS Consensuses of Experts on the Application of Nanopore Sequencing Technology in the Detection of Pathogenic Microorganisms covers targeted sequencing,metagenomic sequencing and whole genome sequencing,and is standardized in terms of sample collection and storage,detection process,bioinformatics analysis and report interpretation;the recommendations are provided for the key issues.关键词
第三代测序/纳米孔测序/病原微生物/危重症感染/临床检验/微生物学诊断Key words
third-generation sequencing/nanopore sequencing/pathogenic microorganisms/critical infection/clinical laboratory tests/microbiological diagnosis分类
医药卫生引用本文复制引用
张述耀,侯铁英,黎小妍,钟诗龙,伍俊妍,黄彬,中国药理学会治疗药物监测研究专业基层委员会,广东省药学会临床治疗精准用药专家委员会,《纳米孔测序在病原微生物检测中的应用专家共识》编写组..纳米孔测序在病原微生物检测中的应用专家共识[J].中国药房,2024,35(14):1673-1682,10.基金项目
广东省科技创新战略专项("大专项+任务清单")项目(No.STKJ202209072) （"大专项+任务清单"）
广东省基础与应用基础研究基金自然科学基金-面上项目(No.2024A1515010633) （No.2024A1515010633）
浙江省科技计划项目"尖兵""领雁"研发攻关计划(No.2023C03068) （No.2023C03068）
中国红十字基金会医学赋能公益专项(No.HSZH202200909) （No.HSZH202200909）
毕节市"揭榜挂帅"重大专项(No.毕科合重大专项[2023]2-2号) （No.毕科合重大专项[2023]2-2号）
