中国药房2024，Vol.35Issue(14)：1715-1721,7.DOI:10.6039/j.issn.1001-0408.2024.14.07
包尔胡特果实的化学成分分析及综合质量评价
Analysis of chemical constituents and comprehensive quality evaluation of Rhamni Songoricae Fructus
摘要
Abstract
OBJECTIVE To identify the chemical constituents of Rhamni Songoricae Fructus and to establish their fingerprints and the method for simultaneous determination of four constituents to comprehensively evaluate the quality of Rhamni Songoricae Fructus.METHODS The chemical constituents in Rhamni Songoricae Fructus were qualitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight tandem mass spectrometry.The fingerprints of 15 batches of Rhamni Songoricae Fructus were established by HPLC and chemometric analysis was performed by using SPSS 26.0 and SIMCA 14.1 software;the contents of quercetin,kaempferol,kaempferide and emodin were determined by the same method.RESULTS A total of 35 constituents were identified,including 28 kinds of flavonoids,5 kinds of anthraquinones and 2 kinds of organic acids.A total of 19 common peaks were identified in the HPLC fingerprints,recognizing quercetin,kaempferol,kaempferide and emodin.The similarities between HPLC fingerprints of 15 batches of samples and control chromatograms were greater than 0.9.The results of cluster analysis showed that 15 batches of samples were divided into 2 classes,of which S1-S5,S7 and S9 were one class and the rest were one class,similar to the results of principal component analysis.The results of the orthogonal partial least squares-discriminant analysis showed that the variable importance projections of peaks No.2,6,1,11(quercetin),3,14,8,10,19(emodin),5 were all greater than 1.The contents of quercetin,kaempferol,kaempferide and emodin ranged from 0.710 to 10.478 mg/g,0.236 to 0.660 mg/g,0.334 to 3.039 mg/g,and 0.261 to 0.504 mg/g.CONCLUSIONS The constructed chemical constituent identification,fingerprint and content determination methods are simple,feasible and reproducible,which combined with chemometric analysis can be used for comprehensive evaluation of the quality of Rhamni Songoricae Fructus.关键词
包尔胡特果实/UPLC-QTOF-MSE/指纹图谱/含量测定/化学成分/质量评价Key words
Rhamni Songoricae Fructus/UPLC-QTOF-MSE/fingerprint/content determination/chemical constituents/quality evaluation分类
医药卫生引用本文复制引用
郭晓宇,滕亮,张晓雪,贾乐,马桂芝..包尔胡特果实的化学成分分析及综合质量评价[J].中国药房,2024,35(14):1715-1721,7.基金项目
新疆维吾尔自治区重大科技专项(No.2022A030-19-3) （No.2022A030-19-3）
