中国药房2024，Vol.35Issue(14)：1727-1731,5.DOI:10.6039/j.issn.1001-0408.2024.14.09
瑶药小钻的特征图谱及其安五脂素含量和抗炎活性研究
Study on characteristic chromatogram of Yao medicine Kadsura longipedunculata and its anwulignan content and anti-inflammatory activity
摘要
Abstract
OBJECTIVE To establish characteristic chromatogram of Yao medicine Kadsura longipedunculata and the method for the content determination of its main component anwulignan,and evaluate the anti-inflammatory activity of anwulignan.METHODS HPLC method was performed with acetonitrile-0.5%phosphoric acid solution as the mobile phase for gradient elution.The characteristic chromatogram of K.longipedunculata was established and similarity was evaluated by Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition).The content of anwulignan in K.longipedunculata was determined.Lipopolysaccharide induced RAW264.7 macrophages were selected as inflammatory cell model to investigate the effects of anwulignan on the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6.RESULTS The similarities of characteristic chromatogram for 10 batches of K.longipedunculata ranged 0.901-0.994,and 9 common peaks were determined;3 components were identified,such as changnan schisantherin E,kadsulactone A,anwulignan.The contents of anwulignan were(0.72±0.05)-(1.21±0.03)mg/g(n=3).Anwulignan of 0.125-0.5 μg/mL greatly decreased the levels of TNF-α,IL-1β and IL-6 in the supernatant of inflammatory model cells(P＜0.05 or P＜0.01).CONCLUSIONS HPLC characteristic chromatogram of K.longipedunculata and the method for the content determination of anwulignan are all established,and anwulignan may be the active ingredient of anti-inflammatory effect in K.longipedunculata.关键词
瑶药小钻/HPLC/特征图谱/质量控制/安五脂素/抗炎作用Key words
Yao medicine Kadsura longipedunculata/HPLC/characteristic chromatogram/quality control/anwulignan/anti-inflammatory action分类
医药卫生引用本文复制引用
唐炳兰,高微,赵成坚,欧春丽,候小利,陈路,莫单丹..瑶药小钻的特征图谱及其安五脂素含量和抗炎活性研究[J].中国药房,2024,35(14):1727-1731,5.基金项目
广西壮族自治区中医药管理局自筹经费科研课题(No.GXZYA20220005) （No.GXZYA20220005）
广西壮族自治区药品监督管理局药品安全科研项目 （）
