中国药理学通报2024，Vol.40Issue(8)：1482-1488,7.DOI:10.12360/CPB202312095
低压低氧致小鼠胃肠应激损伤的研究
Preliminary study on gastrointestinal stress injury induced by hypobaric hypoxia in mice
摘要
Abstract
Aim To study the effects of different peri-ods of hypoxia on gastrointestinal stress in mice by sim-ulating hypoxia environment at high altitude in a low-pressure oxygen chamber.Methods The normal con-trol group and the model group for 1,3 and 5 days were set up according to different periods of hypobaric hypoxia.The intestinal propulsion rate,visceral sensi-tivity and pathological damage of gastrointestinal tract were detected after hypobaric hypoxia treatment.The contents of IL-1 β,IL-6 and TNF-α in gastrointestinal tract and the contents of gastrointestinal hormone,di-amine oxidase and D-lactic acid were detected by en-zyme-linked immunosorbent assay(ELISA).The ex-pressions of tight-junction protein and tight junction in-jury pathway protein in intestinal tissues were detected by Western blot.Results Compared with the control group,the intestinal propulsion rate of the model group was accelerated,and the intestinal sensitivity in-creased.The expression of intestinal mucosal injury markers increased.Gastrointestinal motility inhibiting hormone decreased and gastrointestinal motility promo-ting hormone increased.The expression of tight junc-tion proteins ZO-1,claudin-1,occludin and VASP of tight junction injury-related pathway decreased,while the expression of NF-κB,HIF-1α and VEGF in tight junction injury-related pathway increased.The expres-sions of IL-1 β,IL-6 and TNF-α in gastrointestinal tis-sue increased.The content of pepsin in gastric tissue increased.The injury of gastrointestinal tissue was less in the 1-day group,and more serious in the 3-day and 5-day groups.Conclusions Stress injury occurs in the gastrointestinal tract of mice at different time points in hypoxia and hypoxia environment,and the gastroin-testinal function injury is more serious after three days of exposure,which may be related to the activation of NF-κB/HIF-1α/VEGF/VASP pathway.关键词
低压低氧/小鼠/胃肠应激/小肠推进率/肠黏膜损伤/紧密连接Key words
hypobaric hypoxia/mice/gastrointestinal stress/intestinal propulsion rate/intestinal mucosal in-jury/tight junction分类
医药卫生引用本文复制引用
魏雪,尚慧莹,叶岸平,肖成荣,高月,马增春..低压低氧致小鼠胃肠应激损伤的研究[J].中国药理学通报,2024,40(8):1482-1488,7.基金项目
中医药服务能力培育与提升专项计划重点项目(No 2021ZY009) （No 2021ZY009）
