中国药理学通报2024，Vol.40Issue(8)：1501-1509,9.DOI:10.12360/CPB202402010
当归红芪超滤物对电离辐射引起HUVECs氧化应激和炎症损伤的影响及其机制
Effects of Radix Angelica sinensis and Radix Hedysari ultrafiltration on oxidative stress and inflammatory damage of HUVECs induced by ionizing radiation and its mechanism
摘要
Abstract
Aim To investigate the effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration(RAS-RH)on oxidative stress and inflammatory injury of human umbilical vein endothelial cells(HUVECs)induced by ionizing radiation.Methods The model of HUVECs damage induced by 6 Gy X-rays was estab-lished.HUVECs were treated with different concentra-tions of RAS-RH(100,200,400 μg·L-1).The proliferative activity of HUVECs was detected by CCK-8 method,the structural changes of mitochondria were observed by transmission electron microscope,the level of ROS was detected by DCFH-DA probe,the change of intracellular mitochondrial membrane potential was detected by JC-1 kit,and the apoptosis and cycle were detected by flow cytometry.The contents of IL-6 and TNF-α in cells were detected by ELISA.The activities of MDA,CAT,SOD and GSH-PX were detected by biochemical kit.The gene expression levels of Nrf2,HO-1,NF-κB,eNOS and IL-6 were detected by qRT-PCR,and the expression levels of Nrf2,HO-1,eNOS,NF-κB,p-NF-κB and IL-6 protein were detected by Western blot.Results Compared with the model group,RAS-RH could increase the activity of HUVECs induced by ionizing radiation,decrease the rate of ap-optosis,decrease the level of intracellular ROS,re-duce the injury of intracellular mitochondria,increase the level of mitochondrial membrane potential,promote the expression of Nrf2,HO-1 and eNOS,and inhibit the expression of NF-κB and IL-6.Conclusions RAS-RH has anti-radiation,antioxidant and anti-in-flammatory effects,which may reduce the oxidative stress and inflammatory damage of HUVECs induced by ionizing radiation by activating the activity of Nrf2/HO-1 signal pathway,thus promoting the activity of cell proliferation.关键词
电离辐射/当归红芪超滤物/脐静脉血管内皮细胞/氧化应激/炎症损伤/Nrf2/HO-1信号通路Key words
ionizing radiation/RAS-RH/HUVECs/oxidative stress/inflammatory injury/Nrf2/HO-1 sig-naling pathway分类
医药卫生引用本文复制引用
王丽蓉,王玲云,李晓静,赵会林,霍耀辉,柴辉,刘凯..当归红芪超滤物对电离辐射引起HUVECs氧化应激和炎症损伤的影响及其机制[J].中国药理学通报,2024,40(8):1501-1509,9.基金项目
国家自然科学基金资助项目(No 82260869) （No 82260869）
兰州市人才创新创业项目(No 20219-RC-71) （No 20219-RC-71）
