中国药理学通报2024，Vol.40Issue(8)：1532-1538,7.DOI:10.12360/CPB202412066
胆碱能信号通路及离子通道介导精氨酸对结肠平滑肌收缩的促进作用
Cholinergic signaling pathway and ion channels mediate excitatory effect of arginine on colonic muscle contraction of rats
摘要
Abstract
Aim To investigate the effects of L-argi-nine on spontaneous contraction of rat colon and the relative mechanisms.Methods An organ bath system was use to measure the spontaneous contraction of both longitudinal smooth muscle strips(LMS)and circular muscle strips(CMS).Whole-cell voltage-clamp tech-niques were applied to observe the alterations in cur-rents of L-type voltage-dependent Ca2+channels(VD-CCs),and voltage-gated K+channel(Kv)in single smooth muscle cells(SMCs)from rat colon.Results L-Arginine(1 mmol·L-1)significantly enhanced the spontaneous contraction of both LMS and CMS.The excitatory response to L-arginine was remarkably attenuated by tetrodotoxin(1 mmol·L-1)and atro-pine(1 mmol·L-1),antagonist of muscarinic ACh receptor(mAChR).However,the inhibitor of nitric oxide(NO)synthesis L-NNA(1 mmol·L-1)failed to block the action of L-Arginine.Furthermore,whole-cell patch-clamp recordings showed that L-arginine ac-tivated VDCCs and inhibited Kv channels on SMCs.Conclusions L-Arginine exerts an excitatory effect on colonic motility in a nitric oxide(NO)-independent manner and the stimulatory action of L-arginine is part-ly mediated by mAChR.In addition,VDCCs and Kv channels are both involved in L-arginine-induced exci-tation.关键词
L-精氨酸/结肠/平滑肌/离子通道/动力Key words
L-arginine/colon/smooth muscle/ion channel/motility分类
医药卫生引用本文复制引用
全晓静,王晶,谢丹红,范清,王进海..胆碱能信号通路及离子通道介导精氨酸对结肠平滑肌收缩的促进作用[J].中国药理学通报,2024,40(8):1532-1538,7.基金项目
国家自然科学基金资助项目(No 82100567) （No 82100567）
西安交通大学第二附属医院科研基金青年项目[No YJ(QN)202330] （QN）
