中国药理学通报2024，Vol.40Issue(8)：1565-1572,8.DOI:10.12360/CPB202403045
片仔癀对情志内热诱导流感病毒易感性的作用研究
Effect of Pien Tze Huang on emotional stress-induced influenza virus susceptibility
摘要
Abstract
Aim To evaluate the effect of Pien Tze Huang(PTH)on influenza virus susceptibility in re-straint stress-induced H1N1 influenza susceptibility model in mice with emotional disorders and internal heat,guided by the theory of emotional pathogenesis.Methods Mice were infected with H1N1 influenza vi-rus following 18 h of restraint stress.The signs and weight changes of mice were recorded,and the morbid-ity of mice were analyzed.On the fourth day post viral infection,the lung tissue was collected.The pathologi-cal changes and inflammatory factors in lungs were de-tected by HE staining.The expression of NP was as-sessed by immunohistochemistry and Western blot.The level of lipid peroxidation end products was detected u-sing a commercial kit and western blot.Redox phos-pholipidomics was analyzed in lung tissue by HPLC-MS/MS.Results PTH significantly reduced the mor-tality of influenza-susceptible mice induced by emotion-al stress,inhibited the expression of NP and the re-lease of inflammatory factors,improved inflammation in lung tissue,and alleviated the accumulation of lipid peroxidation end products.Phospholipid oxidation a-nalysis revealed the elevated levels of oxidized phos-pholipid choline and phosphatidylethanolamine in lung tissue of influenza-susceptible mice,which were signif-icantly reduced by PTH administration.Conclusions PTH exhibits promising efficacy in ameliorating influ-enza virus susceptibility induced by internal heat,and its mechanism of action may be related to the regulation of phospholipid peroxidation.关键词
情志应激/片仔癀/清热中药/病毒性肺炎/氧化应激/脂质过氧化Key words
emotional stress/Pien Tze Huang/heat-clearing Chinese medicine/influenza viral pneumonia/oxidative stress/phospholipid peroxidation分类
医药卫生引用本文复制引用
王容,陈新星,黄睿婷,孙万阳,何蓉蓉,李怡芳,黄丰..片仔癀对情志内热诱导流感病毒易感性的作用研究[J].中国药理学通报,2024,40(8):1565-1572,8.基金项目
广东省基础与应用基础研究基金(No 2023B1515040016) （No 2023B1515040016）
广东省粤港澳高校联合实验室(No 2023LSYS002) （No 2023LSYS002）
广州市疾病易感性与中医药防治重点实验室(No 2024A03J090) （No 2024A03J090）
