中国药理学通报2024，Vol.40Issue(8)：1573-1582,10.DOI:10.12360/CPB202311025
基于网络药理学和动物实验探讨慈菇消脂方对非酒精性脂肪性肝炎"炎癌"转化中EGFR/PI3K/AKT信号通路的干预研究
Effect of Cigu Xiaozhi decoction on EGFR/PI3K/AKT signaling pathway in NASH's"inflammatory cancer"transformation based on network pharmacology and animal experiments
摘要
Abstract
Aim To study the main active ingredients,key targets and pathways of Cigu Xiaozhi Decoction(CXD)based on network pharmacology,and to ana-lyze and verify the mechanism of CXD on the transfor-mation of"inflammatory cancer"in non-alcoholic steatohepatitis(NASH)by animal experiments.Meth-ods The potential targets and signaling pathways of CXD in the treatment of NASH"inflammatory carcino-ma"were predicted based on network pharmacology.The mouse model of NASH was induced by methionine-choline deficiency diet(MCD),and CXD and epider-mal growth factor receptor(EGFR)inhibitors were given for 28 days.The mice were killed after the inter-vention,and the liver histopathology of each group was observed by hematoxylin-eosin method(HE).The rel-ative expression levels of EGFR,phosphatidylinositol 3-kinase(PI3K)and protein kinase B(AKT)in liver tissue of mice in each group were detected by Western blot.The contents of interleukin-6(IL-6),interleu-kin-1 β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum were detected by enzyme-linked immunosor-bent assay(ELISA).Results A total of 284 poten-tial active components,159 potential therapeutic tar-gets and 20 key targets of CXD were identified by net-work pharmacological screening.CXD could affect multiple biological processes such as cell proliferation and inflammatory response,involving multiple signa-ling pathways such as tumor and PI3K/AKT.Animal experiments showed that CXD could reduce the levels of IL-6,IL-1β and TNF-α in serum of NASH mice.The relative expression of PI3K and AKT protein in liv-er tissue decreased,and the relative expression of EG-FR protein was increased.Conclusion CXD can reg-ulate EGFR/PI3K/AKT signaling pathway by partici-pating in biological processes such as cell proliferation and inflammatory response,and improve liver tissue injury in NASH mice.关键词
网络药理学/慈菇消脂方/NASH/炎癌/EGFR/PI3K/AKT信号通路Key words
network pharmacology/Cigu Xiaozhi de-coction/NASH/inflammatory cancer/EGFR/PI3K/AKT signaling pathway分类
医药卫生引用本文复制引用
郑兰兰,王莉,郭才,何燕芳,谢娇娇,马燕花..基于网络药理学和动物实验探讨慈菇消脂方对非酒精性脂肪性肝炎"炎癌"转化中EGFR/PI3K/AKT信号通路的干预研究[J].中国药理学通报,2024,40(8):1573-1582,10.基金项目
国家自然科学基金地区基金项目(No 81860821) （No 81860821）
联合科研基金一般项目(No 23JRRA1523) （No 23JRRA1523）
