中国药理学与毒理学杂志2024，Vol.38Issue(7)：504-510,7.DOI:10.3867/j.issn.1000-3002.2024.07.003
牙髓干细胞通过调节氧化应激修复肺微血管内皮细胞低氧损伤
Mesenchymal stem cells inhibit hypoxic damage to rat pulmonary microvascular endothelial cells by regulating oxidative stress
毛壮 1李雪 2王唱垚 3吕琳 1曹虎 1何智超 2余祖胤 1王华4
作者信息
- 1. 军事医学研究院,北京 100850
- 2. 军事医学研究院,北京 100850||安徽医科大学生命科学学院,安徽 合肥 230032
- 3. 军事医学研究院,北京 100850||河北大学生命科学学院,河北 保定 071002
- 4. 军事医学研究院,北京 100850||安徽医科大学生命科学学院,安徽 合肥 230032||河北大学生命科学学院,河北 保定 071002
- 折叠
摘要
Abstract
OBJECTIVE To explore the role and mechanism of dental pulp stem cells(DPSCs)in repairing hypoxic injury to rats pulmonary microvascular endothelial cells(PMVECs).METHODS ①PMVECs were treated with cobalt chloride at 0,10,25,50 and 100 μmol·L-1 for 72 h.CCK-8 was used to detect the cell viability,and the protein levels of hypoxia-inducible factor 1α(HIF-1α),zona occludens small-band protein 1(ZO-1),and occludin(OCLN)were detected by Western blotting.②There was a cell control group,model group,and model+DPSCs group,and the levels of reactive oxygen species(ROS)was detected by immunofluorescence staining after at 24 and 48 h of action.The levels of ZO-1 and OCLN proteins were detected by Western blotting.③ A cell control group,model group,model+DPSC group and model+DPSC cell knockdown superoxide dismutase 1(SOD1)group were set up.The mRNA level of SOD1 was detected by real-time fluorescence quantitative PCR 24 and 48 h later,while the protein levels of ZO-1 and OCLN were detected by Western blotting.RESULTS ① Com-pared with the cell control group,72 h of cobalt chloride 100 μmol·L-1 treatment of PMVECs resulted in a cell survival rate above 80%,a significant increase in the level of HIF-1α protein(P<0.05),a signifi-cant decrease in the levels of ZO-1 and OCLN proteins(P<0.01),and establishment of a model of hypoxic injury in PMVECs.② Compared with the cell control group,the ROS level was significantly higher in the model group(P<0.01).Compared with the model group,the ROS level was significantly lower in the model+DPSCs group(P<0.01),while the levels of ZO-1 and OCLN proteins were signifi-cantly higher in the model+DPSCs group(P<0.05).③ Compared with the DPSC group,ZO-1 and OCLN expressions were significantly decreased after knockdown of SOD1 in DPSCs(P<0.05,P<0.01).CONCLUSIONS DPSCs can repair hypoxic injury to PMVECs,and the anti-oxidative stress capacity of DPSCs plays an important role in hypoxic injury repair of PMVECs.关键词
牙髓干细胞/氧化应激/低氧损伤Key words
mesenchymal stem cells/oxidative stress/hypoxic injury分类
医药卫生引用本文复制引用
毛壮,李雪,王唱垚,吕琳,曹虎,何智超,余祖胤,王华..牙髓干细胞通过调节氧化应激修复肺微血管内皮细胞低氧损伤[J].中国药理学与毒理学杂志,2024,38(7):504-510,7.
