中国药物评价2024，Vol.41Issue(3)：198-203,6.
HPLC-一测多评法测定参葛补肾胶囊中总黄酮醇苷的含量
Simultaneous Determination of Total Flavonol Glycosides in Shenge Bushen Capsule by HPLC-QAMS
摘要
Abstract
Objective:To develop a HPLC method for determination of icariin,epimedin A,epimedin B,epimedin C in Shenge Bush-en capsule.Methods:The chromatographic was performed on a Waters ACQUITY UPLC ® HSS T3 C18column(2.1 × 100 mum,1.8 µm),with acetonitrile(A)-0.05％phosphoric acid solution at the flow rate of 0.3 mL·min-1.Detection wavelength was set at 320 nm,the column temperature was maintained at 30 ℃,and the injection volume was 2 μL.The relative correction factors(fs/k)of the other three components was calculated by slope correction method with icariin as internal reference substance.Results:The linear range of icar-iin,epimedin A,epimedin B and epimedin were 7.13～713.38 μg·mL-1(r=0.999 9),1.28～128.40 μg·mL-1(r=0.999 9),5.11～510.80 µg·mL-1(r=0.999 9),4.36～435.67 μg·mL(r=0.999 9),respectively.The average recoveries were 104.56％,99.69％,100.00％,98.59％,and the RSDs were 1.4％,2.7％,1.2％,2.8％,respectively.By slope correction method,fs/k of epi-medin A,epimedin B and epimedin C were 1.31,1.19,1.17.There were no significance difference in content determination results be-tween two correction methods of QAMS and external standard(P＞0.05).Conclusion:The method is simple,accurate and sensitive,and can be used for the quality control study of total Flavonol Glycosides in Shenge Bushen Capsule.关键词
参葛补肾胶囊/总黄酮醇苷/高效液相色谱/一测多评法Key words
Shenge Bushen capsules/Total flavonol glycosides/High performance liquid chromatography(HPLC)/Quantitative analy-sis of multi-components by single marker(QAMS)分类
医药卫生引用本文复制引用
李成功,胡时先,任宾,潘树球,徐海燕,王涵斌,郝娟..HPLC-一测多评法测定参葛补肾胶囊中总黄酮醇苷的含量[J].中国药物评价,2024,41(3):198-203,6.基金项目
国家重大新药创制科技重大专项资助(2018ZX09731020) （2018ZX09731020）
