中国药物评价2024，Vol.41Issue(3)：204-208,5.
液质联用法定性检测熟大黄配方颗粒中的土大黄苷和番泻苷A
Identification of Rhaponticin and Sennoside A in Stewed Rhubarb Granule by HPLC-MS/MS
王劲 1曲国晶1
作者信息
- 1. 菏泽市食品药品检验检测研究院,山东菏泽 274000
- 折叠
摘要
Abstract
Objective:To found a method for the identification of rhaponticin and sennoside A in Stewed Rhubarb Granule by LC-MS.Methods:An HPLC-MS/MS in MRM mode was adopted.Welch UHPLC XB-C18(2.1 mm × 150 mm,1.8 μm)column was applied with methanol-0.1％phosphoric acid solution as the mobile phase.The Liquid phase flow velocity was 0.3 mL·min-1,The temperature of colum was 35 ℃.The MS/MS system was operated by using an electrospray ionization probe.Scan mode was in multiple reaction ion mo-nitoring(MRM)mode.Results:Limits of detection were 2.0 ng·mL-1 for Rhaponticin and 8.0 ng·mL-1 for Sennoside A.Rhaponticin and Sennoside A weren't found in 8 batches of samples.Conclusion:The method is exclusive and could not only identify the true or false Stewed Rhubarb,but also examine the purity.It's necessary for the quality control of Stewed Rhubarb Granule.关键词
熟大黄配方颗粒/土大黄苷/番泻苷AKey words
Stewed Rhubarb Dispensing Granule/Rhaponticin/Sennoside A分类
医药卫生引用本文复制引用
王劲,曲国晶..液质联用法定性检测熟大黄配方颗粒中的土大黄苷和番泻苷A[J].中国药物评价,2024,41(3):204-208,5.
