中国药物评价2024，Vol.41Issue(3)：217-221,5.
亚抑菌浓度美罗培南对多重耐药鲍曼不动杆菌生物膜作用的研究
Study on the Effect of Meropenem at Subinhibitory Concentration for the Biofilmof Multi-drug Resistant Acinetobacter Baumannii
摘要
Abstract
Objective:To investigate the effect of sub-inhibitory concentrations of meropenem on the biofilm action of multi-drug resist-ant Acinetobacter baumannii(MDR-Ab).Methods:The lowest inhibitory concentration(MIC)of meropenem was measured by broth mi-crodilution.Group A:The biofilm model was constructed with 96-well plate,The mature biofilm was destroyed with meropenem of 1/4 MIC,1/2 MIC and MIC in experimental groups a,b and c,respectively,Group d control group cultured without meropenem for 48 h,96 h,Analysis of bacterial biofilms by crystal violet staining,The OD value of the bacterial biofilm at the wavelength of 560 nm was then measured by a multi-function microplate reader.Group B:The bacterial solution was incubated together with meropenem,Meropenem was added 1/4 MIC,1/2 MIC,MIC to the sub-inhibitory concentration in experimental groups X,Y and Z and cultured MDR-Ab,Group W control group was cultured without meropenem for 48h,96h,Biofilms were determined by the same method.Results:The OD values of the biofilm in group a,b and c in group A and the OD values in the control group d and the OD values in significant difference(P＜0.05),but no significant difference in 96 h(P＞0.05).The OD values in group X,Y,Z and the OD value of control W in 48 h and 96 h in group B(P＜0.05).Conclusion:MDR-Ab has biofilm formation.Group A:meropenem is able to destroy mature biofilm and in-creases with concentration.Group B:MDR-Ab biofilm formation at MIC and 1/2 MIC,may induce biofilm formation at 1/4 MIC.关键词
多重耐药鲍曼不动杆菌(MDR-Ab)/生物膜/亚抑菌浓度/美罗培南Key words
Multiple drug-resistant Acinetobacter baumannii/biofilm/subinhibitory concentration/meropenem分类
医药卫生引用本文复制引用
罗艳,宋沧桑,王波,任宝军,李兴德,张思敏,方甜甜..亚抑菌浓度美罗培南对多重耐药鲍曼不动杆菌生物膜作用的研究[J].中国药物评价,2024,41(3):217-221,5.基金项目
昆明市科技局项目(No.2019KJJH073) （No.2019KJJH073）
昆明市卫生科技人才培养项目[项2020-SW(后备)-36] （后备）
