中国药业2024，Vol.33Issue(14)：57-60,4.DOI:10.3969/j.issn.1006-4931.2024.14.014
依达拉奉对小鼠单核巨噬细胞白血病细胞RAW264.7炎性反应的抑制作用
Inhibitory Effect of Edaravone on the Inflammatory Reactions of Mouse Leukemic Monocyte Macrophage RAW264.7 Cells
摘要
Abstract
Objective To investigate the inhibitory effect of edaravone(EDA)on the inflammatory reactions of mouse leukemic monocyte macrophage RAW264.7 cells.Methods RAW264.7 cells were induced by lipopolysaccharide to replicate the inflammatory cell model.The CCK-8 method was used to detect the cytotoxic effect of EDA.The RAW264.7 cells were divided into the blank group(equal volume of medium),model group(equal volume of medium),and drug group(40 µg/mL EDA).The Griess method was used to detect the nitric oxide(NO)level in cells,the enzyme-linked immunosorbent assay(ELISA)was used to detect the prostaglandin E2(PGE2)and interleukin-1β(IL-1β),interleukin-18(IL-18),tumor necrosis factor-α(TNF-α)and reactive oxygen species(ROS)levels.The real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect the expression levels of IL-1β and IL-18 mRNA in cells.The Western blot was used to detect the expression levels of JAK2/signal transducer and activator of transcription 3(STAT3)pathway-related proteins including JAK2,p-JAK2,STAT3,p-STAT3,IL-1β and IL-18.Results Compared with that at 0 µg/mL,there was no significant change in survival rate of cells at 20,40,80,160 µg/mL EDA(P<0.05).Compared with those in the model group,the NO,PGE2,IL-1β,IL-18,TNF-α and ROS levels in the drug group were significantly lower(P<0.05);the expression levels of IL-1β,IL-18 mRNA and proteins,p-JAK2/JAK2 and p-STAT3/STAT3 in the drug group were significantly lower(P<0.05).Conclusion EDA may inhibit the inflammatory reactions of RAW264.7 cells by inhibiting the activation of the JAK2/STAT3 pathway.关键词
依达拉奉/炎性反应/小鼠单核巨噬细胞白血病细胞RAW264.7/JAK2/STAT3信号通路/作用机制Key words
edaravone/inflammatory reaction/mouse leukemic monocyte macrophage RAW264.7 cells/JAK2/STAT3 signaling pathway/mechanism分类
医药卫生引用本文复制引用
李建芬,刘嘉欣,黄凤蕊,罗球珠..依达拉奉对小鼠单核巨噬细胞白血病细胞RAW264.7炎性反应的抑制作用[J].中国药业,2024,33(14):57-60,4.基金项目
广东省中医药局科研项目[20221152]. （）
