中国药业2024，Vol.33Issue(14)：70-75,6.DOI:10.3969/j.issn.1006-4931.2024.14.017
黄芪桂枝五物汤高效液相色谱指纹图谱及含量测定研究
Study on HPLC Fingerprint and Content Determination of Huangqi Guizhi Wuwu Decoction
摘要
Abstract
Objective To establish a high-performance liquid chromatography(HPLC)fingerprint of Huangqi Guizhi Wuwu Decoction and a content determination method of multi-indicator components.Methods The HPLC method was adopted.The chromatographic column was the Waters SunFireTM C18 column(250 mm×4.6 mm,5 µm),the mobile phase was acetonitrile-0.1% formic acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 254 nm,the column temperature was 40℃,and the injection volume was 10 µL.The HPLC fingerprint of 10 batches of samples was established with cinnamaldehyde peak as a reference.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012A Version)was used for similarity evaluation.The common peaks were identified,and the medicinal herb attribution analysis was conducted.The contents of albiflorin,paeoniflorin,calycosin-7-O-β-D-glucoside,cinnamic acid and cinnamaldehyde were determined by the above chromatographic conditions.Results A total of 17 common peaks in 10 batches of samples were identified,with the similarity greater than 0.990.The verification test showed that the HPLC fingerprint of 10 batches of samples was consistent with the reference fingerprint.The linear ranges of albiflorin,paeoniflorin,calycosin-7-O-β-D-glucoside,cinnamic acid and cinnamaldehyde were 19.0-171.0 µg/mL,61.6-555.0 µg/mL,8.3-75.0 µg/mL,18.0-162.0 µg/mL and 28.4-255.9 µg/mL,respectively.The limit of detection was in the range of 0.1-1.2 µg/mL,and the limit of quantitation was in the range of 0.3-4.9 µg/mL.The RSDs of precision,stability and repeatability tests were all lower than 3.0% .The average recovery rates of the above five components were 99.31%,98.93%,100.89%,98.83%,100.85% with the RSD of 0.25%,0.30%,0.60%,1.99%,0.74% respectively(n=6).Conclusion The method is sample,stable,feasible and repeatable,which can be used for quality control of Huangqi Guizhi Wuwu Decoction.关键词
黄芪桂枝五物汤/高效液相色谱法/指纹图谱/含量测定Key words
Huangqi Guizhi Wuwu Decoction/HPLC/fingerprint/content determination分类
医药卫生引用本文复制引用
张顺宵,陈月,苟小军..黄芪桂枝五物汤高效液相色谱指纹图谱及含量测定研究[J].中国药业,2024,33(14):70-75,6.基金项目
上海市卫生和计划生育委员会科研课题[20184Y0092] （）
上海市宝山区科学技术委员会医学卫生项目[21-E-62]. （）
