CAR-T细胞体内外扩增方法的优化策略OA北大核心CSTPCD

Chimeric antigen receptor gene-modified T(CAR-T)cell immunotherapy is considered as one of the most promising tumor treatments.The number of effector CAR-T cells is a key factor in determining the therapeutic effect of CAR-T cell therapy.The expansion of CAR-T cells in vitro is time and energy-consuming.After transfusion,it's difficult for CAR-T cells to infiltrate into solid tumors,resulting in a significant decrease in the number of CAR-T cells that can effectively inhibit solid tumors.Currently,the amplification of CAR-T cells has problems in enhancing amplification specificity and treatment safety,which hinders the clinical transformation of CAR-T cell therapy.In recent years,achievements in new immunity agonist and their downstream signals have provided more options for CAR-T amplification,and the novel administration methods of immunity agonist have further improved the safety of CAR-T amplification in vivo.This review analyzes the current challenges in CAR-T cell amplification,and systematically expounds the new strategies of CAR-T cell amplification in vitro and in vivo in recent years,providing new thoughts for the efficacy and capacity optimization of CAR-T cell therapy.