中国肿瘤生物治疗杂志2024，Vol.31Issue(7)：675-680,6.DOI:10.3872/j.issn.1007-385x.2024.07.005
高良姜素通过激活cGAS/STING信号通路抑制骨肉瘤MG63细胞的恶性生物学行为
Galangin inhibits the malignant biological behavior of osteosarcoma MG63 cells by activating the cGAS/STING signaling pathway
摘要
Abstract
Objective:To investigate whether galangin(Gal)affects the proliferation,migration,invasion and apoptosis of osteosarcoma MG63 cells by regulating the cGAS/STING signaling pathway.Methods:Human osteosarcoma MG63 cells were cultured in vitro and treated with Gal at concentrations of 0,5,15,25,50,100 and 200 μmol/L for 48 hours,and the effect of Gal on cell viability was detected by CCK-8 method.MG63 cells were divided into the control group(untreated cells),the Gal low concentration group(Gal-L group,treated with 50 μmol/L Gal),the Gal high concentration group(Gal-H group,treated with 100 μmol/L Gal),and the Gal-H+STING inhibitor group(Gal-H+H-151 group,treated with 100 μmol/L Gal+8 μmol/L H-151).EdU staining method,scratch healing test,Transwell chamber method and flow cytometry were used to detect cell proliferation,migration,invasion and apoptosis of cells in each group.Western blot was applied to detect the expression levels of cGAS and STING proteins in cells of each group.Results:Compared with the control group,the proliferation activity,migration,and invasion abilities of the cells in the Gal-L and Gal-H groups were significantly decreased(all P<0.05);the apoptosis rate and the expression levels of cGAS and STING proteins were significantly increased(all P<0.05).Compared with the Gal-H group,the proliferation activity,migration,and invasion abilities of the cells in the Gal-H+H-151 group were significantly increased(all P<0.05);the apoptosis rate and the expression levels of cGAS and STING proteins were significantly decreased(all P<0.05).Conclusion:Gal may inhibit the proliferation,migration and invasion and promote the apoptosis of osteosarcoma cells by activating the cGAS/STING signaling pathway.关键词
高良姜素/骨肉瘤/MG63细胞/增殖/迁移/侵袭,凋亡/cGAS/STING信号通路Key words
galangin(Gal)/osteosarcoma/MG63 cell/proliferation/migration/invasion/apoptosis/cGAS/STING signaling pathway分类
临床医学引用本文复制引用
加鹏飞,余小超,刘小波,李鹏程..高良姜素通过激活cGAS/STING信号通路抑制骨肉瘤MG63细胞的恶性生物学行为[J].中国肿瘤生物治疗杂志,2024,31(7):675-680,6.基金项目
湖北省自然科学基金(No.2020HBA216) （No.2020HBA216）
