中国肿瘤生物治疗杂志2024，Vol.31Issue(7)：681-686,6.DOI:10.3872/j.issn.1007-385x.2024.07.006
京尼平对下咽癌FaDu细胞增殖及线粒体功能的影响
Effects of genipin on the proliferation and mitochondrial function of hypopharyngeal carcinoma FaDu cells
摘要
Abstract
Objective:To explore the effects of UCP2 inhibitor genipin(GEN)on the proliferation and mitochondrial function of human hypopharyngeal carcinoma FaDu cells.Methods:FaDu cells were treated with different concentrations of GEN for 24 hours and divided into the GEN 0 μ mol/L(control)group,the 50 μ mol/L group,the 100 μ mol/L group,the 200 μmol/L group and the 400 μmol/L group.The CCK-8 method was employed to assess cell proliferation,and the DCFH-DA probe and JC-1 flow cytometry were utilized to measure the impact of GEN on reactive oxygen species(ROS)levels and mitochondrial membrane potential in FaDu cells.Laser confocal microscopy was utilized to observe the effect of GEN on the mitochondrial membrane permeability transition pore(MPTP)in FaDu cells.Spectrophotometry was employed to measure lactate levels in cells,and Western blot analysis was conducted to monitor changes in UCP2 protein expression.Results:In comparison with the control group,GEN significantly inhibited the proliferation activity of FaDu cells(P<0.05,P<0.01),reduced the expression of UCP2 protein in cells(P<0.05),decreased the mitochondrial membrane potential(P<0.05,P<0.01)and lactate content(P<0.001),altered mitochondrial membrane permeability,and increased the levels of ROS in cells(P<0.05,P<0.01).Conclusion:GEN modulates the expression of UCP2 in cells,consequently altering their redox potential and mitochondrial function,thus inhibiting the viability and inducing apoptosis in human nasopharyngeal carcinoma FaDu cells.关键词
下咽癌/京尼平/解偶联蛋白2/FaDu细胞/增殖/凋亡/线粒体损伤Key words
hypopharyngeal carcinoma/genipin(GEN)/uncoupling protein 2(UCP2)/FaDu cell/proliferation/apoptosis/mitochondrial damage分类
医药卫生引用本文复制引用
彭瑶,周颖,高宇,刘颖,徐傲枫,张畅,张春晶,于海涛..京尼平对下咽癌FaDu细胞增殖及线粒体功能的影响[J].中国肿瘤生物治疗杂志,2024,31(7):681-686,6.基金项目
齐齐哈尔市科技局科技计划联合引导项目(No.LSFGG-2023031) （No.LSFGG-2023031）
