京尼平对下咽癌FaDu细胞增殖及线粒体功能的影响OA北大核心CSTPCD

Objective:To explore the effects of UCP2 inhibitor genipin(GEN)on the proliferation and mitochondrial function of human hypopharyngeal carcinoma FaDu cells.Methods:FaDu cells were treated with different concentrations of GEN for 24 hours and divided into the GEN 0 μ mol/L(control)group,the 50 μ mol/L group,the 100 μ mol/L group,the 200 μmol/L group and the 400 μmol/L group.The CCK-8 method was employed to assess cell proliferation,and the DCFH-DA probe and JC-1 flow cytometry were utilized to measure the impact of GEN on reactive oxygen species(ROS)levels and mitochondrial membrane potential in FaDu cells.Laser confocal microscopy was utilized to observe the effect of GEN on the mitochondrial membrane permeability transition pore(MPTP)in FaDu cells.Spectrophotometry was employed to measure lactate levels in cells,and Western blot analysis was conducted to monitor changes in UCP2 protein expression.Results:In comparison with the control group,GEN significantly inhibited the proliferation activity of FaDu cells(P<0.05,P<0.01),reduced the expression of UCP2 protein in cells(P<0.05),decreased the mitochondrial membrane potential(P<0.05,P<0.01)and lactate content(P<0.001),altered mitochondrial membrane permeability,and increased the levels of ROS in cells(P<0.05,P<0.01).Conclusion:GEN modulates the expression of UCP2 in cells,consequently altering their redox potential and mitochondrial function,thus inhibiting the viability and inducing apoptosis in human nasopharyngeal carcinoma FaDu cells.