摘要
Abstract
Objective:To investigate the effects of isoliensinine(Iso)on the proliferation,apoptosis and autophagy of colon cancer SW480 cells through PI3K/Akt/mTOR signaling pathway.Methods:Colon cancer SW480 cells were treated with 10,20 and 40 μmol/L Iso,and the effects of Iso on the cell proliferation capacity,apoptosis and expressions of autophagy related proteins LC3Ⅰ,LC3Ⅱ and p62 were detected by CCK-8,flow cytometry and Western blot,respectively.Then,SW480 cells were treated respectively with 20 μmol/L Iso and 25 μmol/L PI3K activator 740 Y-P,and the cells were divided into the control group,the 740 Y-P group,the Iso group and the Iso+740 Y-P group.The effects of 740 Y-P on the apoptosis and the expressions of LC3Ⅰ,LC3Ⅱ,p62,PI3K,p-PI3K,mTOR and p-mTOR proteins in each group were detected by flow cytometry and WB.Results:After treatments with 10,20 and 40 μmol/L Iso,the proliferation capacity of SW480 cells was decreased significantly(all P<0.05);the apoptosis rate was increased significantly(all P<0.05);,the expressions of LC3Ⅱ/LC3Ⅰwere up-regulated significantly(all P<0.05),and the expression of p26 protein was down-regulated significantly(all P<0.05).After treatments with Iso and 740 Y-P,compared with the control group,the apoptosis rate and LC3Ⅱ/LC3Ⅰexpression of the 740 Y-P group were decreased significantly(both P<0.05),while the expressions of p26,p-PI3K/PI3K and p-mTOR/mTOR were increased significantly(all P<0.05).The apoptosis rate and LC3Ⅱ/LC3 expression in the Iso group were increased(both P<0.05)and the expressions of p26,p-PI3K/PI3K and p-mTOR/mTOR were decreased(all P<0.05).Compared with the 740 Y-P group,the apoptosis rate and LC3Ⅱ/LC3Ⅰexpression were increased in the Iso+740 Y-P group(P<0.05),while the expressions of p26,p-PI3K/PI3K and p-mTOR/mTOR were decreased(all P<0.05).Compared with the Iso group,the apoptosis rate and LC3Ⅱ/LC3Ⅰexpression were decreased(both P<0.05),and the expressions of p26,p-PI3K/PI3K,and p-mTOR/mTOR were increased significantly(all P<0.05)in the Iso+740 Y-P group.Conclusion:Iso inhibits the proliferation and induces the apoptosis and autophagy of SW480 cells by inhibiting PI3K/Akt/mTOR signaling pathway.关键词
异莲心碱/结肠癌/SW480细胞/增殖/凋亡/自噬/PI3K/Akt/mTOR信号通路Key words
isoliensinine(Iso)/colon cancer/SW480 cell/proliferation/apoptosis/autophagy/PI3K/Akt/mTOR signaling pathway分类
医药卫生
