异莲心碱通过PI3K/Akt/mTOR信号通路影响结肠癌SW480细胞增殖、凋亡和自噬OA北大核心CSTPCD
Isoliensinine affects the proliferation,apoptosis and autophagy of colon cancer SW480 cells through PI3K/Akt/mTOR signaling pathway
目的:探讨异莲心碱(Iso)通过PI3K/Akt/mTOR信号通路对结肠癌SW480细胞增殖、凋亡和自噬的影响.方法:用10、20和40 μmol/L的Iso处理结肠癌SW480细胞,CCK-8法、流式细胞术和WB法分别检测Iso对细胞增殖活力、凋亡和自噬相关蛋白LC3Ⅰ、LC3Ⅱ、p62表达的影响.然后,用20 μmol/L的Iso和25 μmol/L的PI3K激活剂740 Y-P分别处理SW480细胞,将细胞分为对照组、740 Y-P组、Iso组和Iso+740 Y-P组,流式细胞术、WB法检测Iso和740 Y-P对各组细胞凋亡及细胞中LC3Ⅰ、LC3Ⅱ、p62、PI3K、p-PI3K、mTOR和p-mTOR蛋白表达的影响.结果:10、20和40 μmol/L的Iso处理后,SW480细胞增殖活力均显著下降(均P<0.05),细胞凋亡率均显著升高(均P<0.05),LC3Ⅱ/LC3Ⅰ表达均显著上调(均P<0.05),p26蛋白表达显著下调(P<0.05).Iso和740 Y-P处理后,与对照组相比,740 Y-P组细胞凋亡率、LC3Ⅱ/LC3Ⅰ表达均显著下降(均P<0.05),p26、p-PI3K/PI3K和p-mTOR/mTOR表达均显著升高(均P<0.05);Iso组细胞凋亡率、LC3Ⅱ/LC3Ⅰ表达升高(均P<0.05),p26、p-PI3K/PI3K和p-mTOR/mTOR表达均显著下降(均P<0.05);与740 Y-P组相比,Iso+740 Y-P组细胞凋亡率、LC3Ⅱ/LC3Ⅰ表达升高(P<0.05),p26、p-PI3K/PI3K和p-mTOR/mTOR表达均显著下降(均P<0.05);与Iso组相比,Iso+740 Y-P组细胞凋亡率、LC3Ⅱ/LC3Ⅰ表达下降(均P<0.05),p26、p-PI3K/PI3K和p-mTOR/mTOR表达均显著升高(均P<0.05).结论:Iso通过抑制PI3K/Akt/mTOR信号通路抑制结肠癌SW480细胞增殖并诱导细胞凋亡和自噬.
Objective:To investigate the effects of isoliensinine(Iso)on the proliferation,apoptosis and autophagy of colon cancer SW480 cells through PI3K/Akt/mTOR signaling pathway.Methods:Colon cancer SW480 cells were treated with 10,20 and 40 μmol/L Iso,and the effects of Iso on the cell proliferation capacity,apoptosis and expressions of autophagy related proteins LC3Ⅰ,LC3Ⅱ and p62 were detected by CCK-8,flow cytometry and Western blot,respectively.Then,SW480 cells were treated respectively with 20 μmol/L Iso and 25 μmol/L PI3K activator 740 Y-P,and the cells were divided into the control group,the 740 Y-P group,the Iso group and the Iso+740 Y-P group.The effects of 740 Y-P on the apoptosis and the expressions of LC3Ⅰ,LC3Ⅱ,p62,PI3K,p-PI3K,mTOR and p-mTOR proteins in each group were detected by flow cytometry and WB.Results:After treatments with 10,20 and 40 μmol/L Iso,the proliferation capacity of SW480 cells was decreased significantly(all P<0.05);the apoptosis rate was increased significantly(all P<0.05);,the expressions of LC3Ⅱ/LC3Ⅰwere up-regulated significantly(all P<0.05),and the expression of p26 protein was down-regulated significantly(all P<0.05).After treatments with Iso and 740 Y-P,compared with the control group,the apoptosis rate and LC3Ⅱ/LC3Ⅰexpression of the 740 Y-P group were decreased significantly(both P<0.05),while the expressions of p26,p-PI3K/PI3K and p-mTOR/mTOR were increased significantly(all P<0.05).The apoptosis rate and LC3Ⅱ/LC3 expression in the Iso group were increased(both P<0.05)and the expressions of p26,p-PI3K/PI3K and p-mTOR/mTOR were decreased(all P<0.05).Compared with the 740 Y-P group,the apoptosis rate and LC3Ⅱ/LC3Ⅰexpression were increased in the Iso+740 Y-P group(P<0.05),while the expressions of p26,p-PI3K/PI3K and p-mTOR/mTOR were decreased(all P<0.05).Compared with the Iso group,the apoptosis rate and LC3Ⅱ/LC3Ⅰexpression were decreased(both P<0.05),and the expressions of p26,p-PI3K/PI3K,and p-mTOR/mTOR were increased significantly(all P<0.05)in the Iso+740 Y-P group.Conclusion:Iso inhibits the proliferation and induces the apoptosis and autophagy of SW480 cells by inhibiting PI3K/Akt/mTOR signaling pathway.
王湘宁;张金华;江娜;刘志平;徐莹
武汉市汉口医院 消化内科,湖北 武汉 430000
临床医学
异莲心碱结肠癌SW480细胞增殖凋亡自噬PI3K/Akt/mTOR信号通路
isoliensinine(Iso)colon cancerSW480 cellproliferationapoptosisautophagyPI3K/Akt/mTOR signaling pathway
《中国肿瘤生物治疗杂志》 2024 (007)
694-699 / 6
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